菌落總數(shù):Aerobic Plate Count,食品檢樣經(jīng)過(guò)處理,在一定條件下培養(yǎng)后(如培基、溫度、時(shí)間、pH、需氧性質(zhì)等)所得1mL(g)檢樣中形成菌落的總數(shù)。只包括一群在平板技術(shù)瓊脂上生長(zhǎng)發(fā)育的嗜中溫需氧或兼性厭氧菌的菌落總數(shù)。菌落總數(shù)不等于細(xì)菌總數(shù)。
菌落:Colony,單個(gè)微生物在適宜固體培養(yǎng)基表面或內(nèi)部生長(zhǎng)繁殖到一定程度;形成肉眼可見(jiàn)有 一定細(xì)菌群落。
CFU: Colony Forming Units,菌落形成單位。
菌落計(jì)數(shù)方法:選取菌落數(shù)在30~300cfu之間,無(wú)蔓延生長(zhǎng)的平板計(jì)數(shù)。低于30的記錄具體菌落數(shù),大于300的可記錄為多不可計(jì),每個(gè)稀釋度采用兩個(gè)平行的均數(shù)。有較大片狀菌落生長(zhǎng)者不宜采用,若片狀小于平板一半時(shí)且余部均勻分布,則以半個(gè)平板菌落數(shù)2倍。報(bào)告無(wú)明顯界限鏈狀菌落每條單鏈視為一個(gè)菌落。
凝集反應(yīng)
顆粒性抗原(細(xì)菌、紅細(xì)胞等)與相應(yīng)抗體結(jié)合,在電解質(zhì)參與下所形成的肉眼可見(jiàn)的凝集現(xiàn)象,稱為凝集反應(yīng)(Agglutination reaction)。其中的抗原稱為凝集原,抗體稱為凝集素。直接凝集反應(yīng)
顆粒性抗原如細(xì)菌和細(xì)胞與相應(yīng)抗體直接結(jié)合所出現(xiàn)的反應(yīng)。
Total number of colonies: Aerobic Plate Count, processed food inspection samples, under certain conditions after training (such as prudential, temperature, time, pH, Aerobic properties, etc.) from 1 ml (g) the total number of colonies formed in the test samples. The total number of colonies of mesophilic aerobes or facultative anaerobes growing and developing on AGAR plate technology is included. The total number of colonies is not equal to the total number of bacteria.
Colony: Colony, where a single microorganism grows to a certain extent on or within a suitable solid medium; There is a certain community of bacteria visible to the naked eye.
CFU: Colony Forming Units, Colony Forming Units.
Colony counting method: the number of colonies was selected between 30 and 300cfu, and the number of plates with no spreading growth was selected. The number of specific colonies recorded below 30 is uncountable, and the number of colonies recorded above 300 is uncountable. Two parallel mean values are adopted for each dilution degree. It is not suitable for people with large pellets to grow. If the pellets are less than half of the plate and the rest are evenly distributed, the number of colonies of half plate is twice. There is no significant limit reported for each single chain of streptococcus colonies as a colony.
agglutination
Granular antigen (bacteria, red blood cells, etc.) binds to the corresponding antibodies, and the visible Agglutination phenomenon formed under the participation of electrolytes is called Agglutination reaction. The antigen is called agglutinin, and the antibody is called agglutinin. Direct agglutination
Reactions in which granular antigens such as bacteria and cells bind directly to the corresponding antibodies.