磷酸鹽緩沖液pH6.0的配置方法
發(fā)布時間：
2022-12-27
作者：
培養(yǎng)基購買 日水生物
磷酸鹽緩沖液（pH6.0）用于樣品的稀釋、制備（中國藥典）樣品制備；英文名 pH6.0 PBS Broth；編號Cat. No. 10135-1；信息來自日水生物培養(yǎng)基。
磷酸鹽緩沖液（pH6.0）的配方 克/升
磷酸二氫鉀 8.0克
磷酸氫二鉀 2.0克
pH6.0±0.1(25攝氏度)
磷酸鹽緩沖液（pH6.0）的配制方法:
稱取本品10.0g于1升蒸餾水或去離子水中（可按比例增加或減少配制量），加熱煮沸至完全溶解，分裝，115攝氏度高壓滅菌30分鐘,備用。
Phosphate buffer (pH6.0) was used for sample dilution and preparation (Chinese pharmacopoeia) sample preparation; PH6.0 PBS Broth; No. Cat. No. 10135-1; The information came from the sun aquatic culture medium.
PH6.0 PBS Broth Formula G /L
8.0 g Monopotassium phosphate
Dipotassium Phosphate 2.0 g
PH6.0 + / - 0.1 (25 ℃)
Preparation method of phosphate buffer (pH6.0) :
Weigh 10.0g of the product in 1L distilled water or deionized water (the amount of preparation can be increased or decreased in proportion), boil the product until it is completely dissolved, divide into separate containers, and autoclaviate at 115℃ for 30 minutes, set aside.