緩沖動力-硝酸鹽培養(yǎng)基基礎(chǔ)動力-硝酸鹽培養(yǎng)基B法基礎(chǔ)配方
發(fā)布時間：
2022-12-27
作者：
緩沖動力-硝酸鹽培養(yǎng)基基礎(chǔ)動力-硝酸鹽培養(yǎng)基B法基礎(chǔ)配制方法：稱取本品23.5g于1L蒸餾水或去離子水中，加入5mL甘油[可按比例增加或減少配制量]，加熱煮沸至完全溶解，分裝，121℃高壓滅菌15分鐘,滅菌結(jié)束后請搖勻，以防瓊脂沉淀于器皿底部，備用。
Power-nitrate Medium Formula[配方]： g/L
Beef Extract[牛肉浸粉]：3.0g
Peptone [蛋白胨[：5.0g
Potassium Nitrate[硝酸鉀]：5.0g
Disodium Hydrogen Phosphate[磷酸氫二鈉]：2.5g
Galactose[半乳糖]：5.0g
Agar[瓊脂]：3.0g
pH7.3±0.1[25℃]
Buffer power-nitrate medium basic power-nitrate medium B basic preparation method: weigh 23.5g in 1L distilled water or deionized water, add 5mL glycerol [can increase or reduce the amount of preparation in proportion], heat and boil until completely dissolved, sub-packed, high-pressure sterilization at 121 C for 15 minutes, shake well after sterilization to prevent agar Grease is deposited at the bottom of the utensils.
Power-nitrate Medium Formula[formula]:g/L
Beef Extract[beef dip powder]:3.0g
Peptone [peptone [:5.0g
Potassium Nitrate[potassium nitrate]:5.0g
Disodium Hydrogen Phosphate[hydrogen phosphate two sodium]:2.5g
Galactose[galactose]:5.0g
Agar[agar]:3.0g
PH7.3 + 0.1[25 C]