底層培養(yǎng)基(Ames 試驗(yàn))干粉的配方是什么？
發(fā)布時(shí)間：
2022-12-27
作者：
日水生物
底層培養(yǎng)基(Ames 試驗(yàn))的配制方法: 稱取本品50.7g于1L蒸餾水或去離子水中（可按比例增加或減少配制量），加熱至完全溶解，分裝，115℃高壓滅菌20分鐘,滅菌結(jié)束后請搖勻,以防瓊脂沉積于器皿底部而凝固，備用。
the underlying medium Formula(配方)： g/L
Microcosmic Salt\磷酸氫鈉銨:3.5g
Citric Acid\檸檬酸:2.0g
Magnesium Sulfate\硫酸鎂:0.2g
Dipotassium phosphate\磷酸氫二鉀:10.0g
Dextrose\葡萄糖:20.0g
Agar\瓊脂:15.0g
The preparation method of the bottom culture medium (Ames test) the underlying medium: call this product 50.7g in 1L distilled water or deionized water (can increase or reduce the dosage in proportion), heat to completely dissolve, pack, sterilize at 115 degrees centigrade for 20 minutes, after sterilization, please shake well in order to prevent agar from depositing at the bottom of the utensils.
Formula (formula):g/L
Ammonium hydrogen phosphate: 3.5g
Citric acid: 2.0g
Magnesium Sulfate: 0.2g
Two potassium hydrogen phosphate: 10.0g
Glucose: 20.0g
Agar: 15.0g