溴甲酚紫乳糖瓊脂干粉培養(yǎng)基的配制
發(fā)布時間：
2022-12-27
作者：
日水生物
溴甲酚紫乳糖瓊脂干粉培養(yǎng)基配制方法: 稱取本品31.0g于1L蒸餾水或去離子水中，加熱煮沸至完全溶解，分裝,121℃高壓滅菌15分鐘,滅菌結(jié)束后請搖勻，以防瓊脂沉積于器皿底部而凝固，備用。
Formula/配方： g/L
Tryptone/胰蛋白胨:5.0g
Lactose/乳糖:10.0g
Beef Extract/牛肉浸粉:3.0g
Bromcresol Purple/溴甲酚紫:0.025g
Agar /瓊脂:13.0g
pH7.0±0.2 /25℃
Preparation method: call this product 31.0g in 1L distilled water or deionized water, heat and boil to completely dissolve, pack, sterilize at 121 centigrade for 15 minutes, after sterilization, please shake well in order to prevent agar from depositing at the bottom of the utensils.
Formula: g/L
Tryptone: 5.0g
Lactose: 10.0g
Beef dipping: 3.0g
Bromo cresol violet: 0.025g
Agar: 13.0g
PH7.0 + 0.2 /25 C