改良頭孢哌酮麥康凱瓊脂（CMA）基礎(chǔ)的配方
發(fā)布時間：
2022-12-27
作者：
日水生物
改良頭孢哌酮麥康凱瓊脂（CMA）基礎(chǔ)配制方法: 稱取本品67.0g于1L蒸餾水或去離子水中/可按比例增加或減少配制量），加熱煮沸至完全溶解，分裝，115℃高壓滅菌15分鐘,冷卻至50-55℃，每100mL培養(yǎng)基中加入1支20222/頭孢哌酮溶液，1.6mg/支），搖勻，備用。
Formula/配方)： g/L
Peptone /蛋白胨:17.0g
Peptone/胨:3.0g
Bile Salts/牛膽鹽:5.0g
Sodium Chloride/氯化鈉:5.0g
Lactose/乳糖:10.0g
Glucose/葡萄糖:10.0g
Crystal Violet/結(jié)晶紫:0.001g
Neutral Red/中性紅:0.025g
Agar/瓊脂:17.0g
pH7.2±0.2/25℃)
Preparation method: This product is called 67.0g in 1L distilled water or deionized water / can increase or reduce the dosage in proportion), heat and boil to completely dissolve, pack, sterilize at 115 centigrade for 15 minutes, cool to 50-55 C, add 1 20222/ cefoperazone solution per 100mL medium, 1.6mg/ branch, shake up and spare.
Formula: g/L
Peptone: 17.0g
Peptone: 3.0g
Bovine bile salt: 5.0g
Sodium chloride: 5.0g
Lactose: 10.0g
Glucose: 10.0g
Crystal violet: 0.001g
Neutral red: 0.025g
Agar: 17.0g
PH7.2 + 0.2/25 C)