陰溝腸桿菌分離瓊脂配方制備
發(fā)布時間:
2022-12-27
作者:
日水生物
陰溝腸桿菌分離瓊脂配制方法: 稱取本品49.5g于1L蒸餾水或去離子水中/可按比例增加或減少配置量),攪拌煮沸溶解1分鐘,冷卻至50-55℃傾注平板。
Formula/配方:g/L
Mixed peptone/混合蛋白胨:23.0g
Raffinose/棉子糖:10.0g
Sodium Chloride/氯化鈉:5.0g
Bile Salts No.3/3號膽鹽:1.5g
Bromthymol Blue/溴麝香草酚藍(lán):0.03g
Crystal Violet/結(jié)晶紫:0.002g
Agar/瓊脂:10.0g
pH7.4±0.1/25℃
Preparation method: the product is called 49.5g in 1L distilled water or deionized water / can increase or reduce the configuration in proportion), stirring and dissolving for 1 minutes, cooling to 50-55 centigrade to pour the plate.
Formula: g/L
Mixed peptone: 23.0g
Raffinose: 10.0g
Sodium chloride: 5.0g
No. 3 bile salt: 1.5g
Bromothymol blue: 0.03g
Crystal violet: 0.002g
Agar: 10.0g
PH7.4 + 0.1/25 C