α -甲基-D-葡萄糖苷測(cè)定培養(yǎng)基的配方是什么
發(fā)布時(shí)間：
2022-12-27
作者：
日水生物
α -甲基-D-葡萄糖苷測(cè)定培養(yǎng)基配制方法：稱取本品50.33g于1L蒸餾水或去離子水中/可按比例增加或減少配制量）,加熱煮沸至完全溶解，分裝，121℃高壓滅菌3分鐘,滅菌結(jié)束后請(qǐng)搖勻，以防瓊脂沉積于器皿底部而凝固，備用。
Formula/配方)： g/L
Peptone/蛋白胨：20.0g
Beef Extract/牛肉膏粉：5.0g
Sodium Chloride/氯化鈉：5.0g
Sodium lauryl Sulfate/月桂基硫酸鈉：0.25g
5- bromine -4- chloride 3 α-D Glucoside /5-溴-4-氯3吲哚α-D葡萄糖苷：0.08g
Agar/瓊脂：20.0g
pH7.3±0.1/25℃)
The preparation method of alpha methyl -D- glucoside determination culture medium: call this product 50.33g in 1L distilled water or deionized water / can increase or reduce the dosage in proportion), heat and boil to completely dissolve, pack, sterilize at 121 centigrade for 3 minutes, after sterilization, please shake well in order to prevent agar from depositing at the bottom of the utensils.
Formula: g/L
Peptone: 20.0g
Beef paste: 5.0g
Sodium chloride: 5.0g
Lauryl sodium sulfate: 0.25g
5- bromine -4- chloride 3 indole alpha -D glucoside: 0.08g
Agar: 20.0g
PH7.3 + 0.1/25 C