品紅亞硫酸鈉液體培養(yǎng)基配制方法
發(fā)布時間：
2022-12-27
作者：
日水生物
品紅亞硫酸鈉液體培養(yǎng)基配制方法: 稱取本品3.95g于100ml蒸餾水或去離子水中，添加無水乙醇2ml,115℃高壓滅菌20分鐘,備用。
Formula(配方)： g/L
Peptone/蛋白胨：10.0g
Yeast Extract/酵母浸粉：5.0g
Beef Extract/牛肉浸粉：5.0g
Lactose/乳糖：10.0g
Dipotassium phosphate /磷酸氫二鉀：3.5g
Sodium Sulfite/亞硫酸鈉：5.0g
Basic Fuchsin/堿性品紅：1.0g
pH7.3±0.1(25℃)
The preparation method of the liquid culture medium of fuchsin sodium sulfite: called 3.95g in 100ml distilled water or deionized water, adding anhydrous ethanol 2ml, sterilizing at 115 C for 20 minutes, and being spare.
Formula: g/L
Peptone: 10.0g
Yeast extract: 5.0g
Beef dipping: 5.0g
Lactose: 10.0g
Two potassium hydrogen phosphate: 3.5g
Sodium sulfite: 5.0g
Basic fuchsin: 1.0g
PH7.3 + 0.1 (25 C)