腦-心浸萃瓊脂培養(yǎng)基的配方說(shuō)明詳解
發(fā)布時(shí)間：
2022-12-27
作者：
日水生物
腦-心浸萃瓊脂培養(yǎng)基配制方法: 稱(chēng)取本品52.0g于1L蒸餾水或去離子水中可按比例增加或減少配制量），加熱溶解，分裝，121℃高壓滅菌15分鐘,滅菌結(jié)束后請(qǐng)搖勻，以防瓊脂沉積于器皿底部而凝固，備用。
Formula腦-心浸萃瓊脂培養(yǎng)基配方： g/L
Proteose示蛋白胨：10.0g
Sodium Chloride氯化鈉：5.0g
Disodium Hydrogen Phosphate
磷酸氫二鈉：2.5g
Dextrose葡萄糖：2.0g
Heart Extract牛心浸粉：9.8g
Brains Extract牛腦浸粉：7.7g
Agar瓊脂：15.0g
pH7.4±0.225℃
Preparation method: take this product 52.0g in 1L distilled water or deionized water to increase or reduce the amount of preparation), heat dissolve, dissolve, sterilization at 121 degrees centigrade for 15 minutes, after sterilization, please shake well in order to prevent agar from depositing at the bottom of the utensils and solidify. Spare.
Formula formula):g/L
Proteose peptone: 10.0g
Sodium Chloride sodium chloride: 5.0g
Disodium Hydrogen Phosphate
Two sodium hydrogen phosphate: 2.5G
Dextrose glucose: 2.0g
Heart Extract cow heart dipping powder: 9.8g
Brains Extract bovine brain dipping powder: 7.7g
Agar agar: 15.0g
PH7.4 + 0.225 C