KF鏈球菌瓊脂培養(yǎng)基的配制方法 配置
發(fā)布時(shí)間：
2022-12-27
作者：
日水生物
KF鏈球菌瓊脂培養(yǎng)基配制方法: 稱取本品76.5g于1L蒸餾水或去離子水中可按比例增加或減少配制量），加熱煮沸1分鐘使完全溶解，繼續(xù)加熱5分鐘，冷卻至50℃左右傾注平皿。
FormulaKF鏈球菌瓊脂培養(yǎng)基配方 ： g/L
Proteose Peptone No. 33號(hào)月示胨：10.0g
Maltose麥芽糖：20.0g
Lactose乳糖：1.0g
Yeast Extract酵母浸粉：10.0g
Sodium Chloride氯化鈉：5.0g
Sodium Azide 疊氮化鈉：0.4g
Sodium Glycerophosphate
甘油磷酸鈉：10.0g
Bromcresol Purple溴甲酚紫：0.015g
TTCTTC：0.1g
Agar瓊脂：20.0g
pH7.2±0.225℃
Preparation method: it is called 76.5g in 1L distilled water or deionized water to increase or reduce the amount of preparation according to proportion), heat and boil for 1 minutes to completely dissolve, continue to heat for 5 minutes, cooling to about 50 degrees centigrade.
Formula formula):g/L
Proteose Peptone No. 33 month peptone: 10.0g
Maltose maltose: 20.0g
Lactose lactose: 1.0g
Yeast Extract yeast extract powder: 10.0g
Sodium Chloride sodium chloride: 5.0g
Sodium Azide sodium azide: 0.4g
Sodium Glycerophosphate
Sodium glyceryl phosphate: 10.0g
Bromcresol Purple bromo cresol violet: 0.015g
TTCTTC:0.1g
Agar agar: 20.0g
PH7.2 + 0.225 C