慶大霉素瓊脂基礎(chǔ)培養(yǎng)基的配制
發(fā)布時間:
2022-12-27
作者:
日水生物
慶大霉素瓊脂基礎(chǔ)培養(yǎng)基的配制方法:
稱取本品56.0g于1L蒸餾水或去離子水中(可按比例增加或減少配制量),121℃高壓滅菌15分鐘,冷卻至50—55℃,每100ml培養(yǎng)基中添加1支20543,混勻,傾注平板。
Formula(慶大霉素瓊脂基礎(chǔ)培養(yǎng)基配方): g/L
Peptone(蛋白胨) 10.0g
Beef Extract(牛肉浸出粉) 3.0g
Sodium Sulfite(亞硫酸鈉) 3.0g
Sodium Citrate (檸檬酸鈉) 10.0g
Sodium Chloride(氯化鈉) 5.0g
Sucrose(蔗糖) 10.0g
Agar(瓊脂) 15.0g
pH 8.4±0.1(25℃)
Preparation method:
According to take this product 56.0 g in 1 l of distilled water or deionized water (can be in proportion to increase or decrease preparation), 121 ℃ high pressure sterilization for 15 minutes, cool to 50 to 55 ℃, each 100 ml, 1-20543 is added to the medium blender, pour into the tablet.
Formula: g/L
Peptone (Peptone) 10.0g
Beef Extract 3.0g
Sodium Sulfite 3.0g
Sodium Citrate (Sodium Citrate) is 10.0g
Sodium Chloride (nacl) 5.0 g
Sucrose (Sucrose) 10.0g
Agar (Agar) 15.0g
PH 8.4 + / - 0.1 (25 ℃)