改良 Y 培養(yǎng)基
發(fā)布時(shí)間：
2022-12-27
作者：
日水生物
改良 Y 培養(yǎng)基的配方
配制方法:
稱取本品67.0g于1L蒸餾水或去離子水
中（可按比例增加或減少配制量）,加熱煮
沸至完全溶解，分裝，121℃高壓滅菌15分鐘,滅菌結(jié)束后請(qǐng)搖勻，以防瓊脂沉淀于器
皿底部而凝固，備用。
Formula(配方)： g/L
Peptone （蛋白胨） 15.0g
Sodium Chloride(氯化鈉) 5.0g
Lactose（乳糖） 10.0g
Sodium oxalate（草酸鈉） 2.0g
Sodium Desoxycholate（去氧膽酸鈉） 6.0 g
Bile Salts No.3(3號(hào)膽鹽) 5.0g
Sodium pyruvate(丙酮酸鈉) 2.0g
Rose Bengal(孟加拉紅) 40.0mg
Hydrolysis of Casein(水解酪蛋白) 5.0g
Agar（瓊脂） 17.0g
pH7.4±0.1(25℃)
Formulation of modified Y medium
Preparation method:
Weigh 67.0g of this product in 1L distilled water or deionized water
Medium (can increase or decrease proportionally), heat and cook
Boiling until completely dissolved, repackaging, 121 ℃ high pressure sterilization for 15 minutes, please shake after sterilization, to prevent AGAR precipitation in the device
Freeze the bottom of the dish. Set aside.
Formula: g/L
Peptone (Peptone) 15.0g
Sodium Chloride (nacl) 5.0 g
Lactose (Lactose) 10.0g
Sodium oxalate (Sodium oxalate) 2.0g
Sodium Desoxycholate 6.0 g
Bile Salts no.3 (Bile Salts no.3) 5.0g
Sodium pyruvate(Sodium pyruvate) 2.0g
Rose Bengal(Bengal red) 40.0mg
Hydrolysis of Casein(hydrolyzed Casein) 5.0g
Agar (Agar) 17.0g
PH7.4 slightly + / - 0.1 (25 ℃)