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玉米粉瓊脂培養(yǎng)基的配方和使用方法

發(fā)布時(shí)間:

2022-12-27

作者:

日水生物


配制方法:
   稱(chēng)取本品17.0g于1L蒸餾水或去離子水中(如有需要,可添加1%吐溫80或1%葡萄糖),加熱煮沸至完全溶解,分裝,121℃ 高壓滅菌15分鐘,滅菌結(jié)束后請(qǐng)搖勻,以防瓊脂沉積于器皿底部而凝固,備用。
  
 
Formula(配方): g/L
Corn Meal  Infusion(玉米浸粉)          2.0g          
Agar(瓊脂)                                           15.0g                           
pH6.0±0.2 (25℃)
 
 

 

Preparation method:
According to take this product 17.0 g in 1 l of distilled water or deionized water (if necessary, you can add 1% twain 80 or 1% glucose), heating boiling until completely dissolved, repackaging, 121 ℃ high pressure sterilization for 15 minutes, please shake after sterilization, to prevent AGAR deposited on the bottom of the vessel and solidification, and set aside.
 
 
Formula: g/L
Corn Meal Infusion 2.0g
Agar (Agar) 15.0g
PH6.0 + / - 0.2 (25 ℃)