Middle Brook 7H10 瓊脂英文名稱:Middle Brook 7H10 Agar Base
產(chǎn)品編號:12137
產(chǎn)品規(guī)格:250g/瓶
產(chǎn)品用途:用于分枝桿菌的分離培養(yǎng)
Formula(Middle Brook 7H10 瓊脂配方): g/L
Ammonium Sulfate(硫酸銨) 0.5g
L-Sodium Glutamate (L-谷氨酸鈉) 0.5g
Sodium Citrate (檸檬酸鈉) 0.4g
Vitamin B6(維生素B6) 1.0mg
Biotin(生物素) 0.5mg
Disodium Hydrogen Phosphate (磷酸氫二鈉) 1.5g
Monopotassium Phosphate(磷酸二氫鉀) 1.5g
Ferric Ammonium Citrate(檸檬酸鐵銨) 0.04g
Magnesium Sulfate(硫酸鎂) 25mg
Calcium Chloride (氯化鈣) 0.5mg
Zinc Sulfate(硫酸鋅) 1.0mg
Copper Sulphate(硫酸銅) 1.0mg
Malachite Green(孔雀綠) 0.25mg
Agar(瓊脂) 15.0g
pH6.6±0.2(25℃)
配制方法:
稱取本品3.8g于180ml蒸餾水或去離子水中,添加一支21702(甘油),加熱煮沸溶解,121℃高壓滅菌10分鐘,冷卻至50~55℃時無菌操作添加一支22114(Middlebrook OADC
增菌液),備用。
Middle Brook 7H10 Agar Base
Product number: 12137
Product specification: 250g/ bottle
Application: for isolation and culture of mycobacterium
Formula: g/L
Ammonium Sulfate (Ammonium Sulfate) 0.5 g
L-Sodium Glutamate (L- Glutamate Sodium) 0.5g
Sodium Citrate (Sodium Citrate) is 0.4g
Vitamin B6 (Vitamin B6) 1.0mg
Biotin (Biotin) 0.5mg
Disodium Hydrogen Phosphate, Disodium Hydrogen Phosphate) 1.5 g
Monopotassium Phosphate (KDP) 1.5 g
Ferric Ammonium Citrate (Ferric Ammonium Citrate) 0.04 g
Magnesium Sulfate, Magnesium Sulfate) 25 mg
Calcium Chloride (cacl2) 0.5 mg
Zinc Sulfate (Zinc Sulfate) 1.0mg
Copper Sulphate (Copper sulfate) 1.0 mg
Malachite Green (Malachite Green) 0.25mg
Agar (Agar) 15.0g
PH6.6 + / - 0.2 (25 ℃)
Preparation method:
Said get a 3.8 g of the product in 180 ml of distilled water or deionized water, add a 21702 (glycerin), heat to boil to dissolve, 121 ℃ and high pressure sterilization for 10 minutes, cooled to 50 sterile operation to add a 22114 ~ 55 ℃ (Middlebrook OADC
Germicidal fluid), set aside.