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配置木本植物用培養(yǎng)基（WPM）配方

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2022-12-27

作者：

配置木本植物用培養(yǎng)基（WPM）配方

英文名稱：Wood Plant Medium      
產(chǎn)品編號：11935
產(chǎn)品規(guī)格：250g/瓶
產(chǎn)品用途：用于植物組織培養(yǎng)
                                                                                                                   
Formula(配方)： g/L
Nutrient Element（營養(yǎng)元素） 　      2.5g
Sucrose（蔗糖）               20.0g
Agar（瓊脂）                 7.0g
      
培養(yǎng)基的配制方法:
     稱取本品29.5g于1L蒸餾水或去離子水中,調(diào)節(jié)pH使滅菌后pH5.2±0.1(25℃),
分裝,115℃高壓滅菌30分鐘，滅菌結(jié)束后請搖勻，以防瓊脂沉積于器皿底部而凝固，
備用。
 ; mso-bidi-font-family:Times New Roman; font-size:10.5000pt; mso-font-kerning:1.0000pt; " >　               4.74g

Sucrose(蔗糖)                                50.0g
      
配制方法:
    稱取本品54.74g于1L蒸餾水或去離子水中，加熱煮沸溶解，調(diào)節(jié)pH使滅菌后至pH5.6±0.2(25℃)，分裝，115℃高壓滅菌30分鐘，備用。

 

Wood Plant Medium
Product number: 11935
Product specification: 250g/ bottle
USES: for plant tissue culture
 
Formula: g/L
Nutrient Element (nutrients) 2.5 g
Sucrose (Sucrose) 20.0g
Agar (Agar) 7.0g
 
Preparation method:
Said to take this product 29.5 g in 1 l of distilled water or deionized water, adjust pH after the sterilization pH5.2 + / - 0.1 (25 ℃),
Packaging of 115 ℃ high pressure sterilization for 30 minutes, please shake after sterilization, to prevent AGAR deposited on the bottom of the vessel and solidification,
Set aside.
 ; The oledata.mso bidi - the font-family: Times New Roman; The font - size: 10.5000 pt; The oledata.mso - the font - kerning: 1.0000 pt; "> 4.74 g
 
Sucrose(Sucrose) 50.0g
 
Preparation method:
According to take this product 54.74 g in 1 l of distilled water or deionized water, heating boiling dissolved, adjust pH after sterilization to pH5.6 + / - 0.2 (25 ℃), repackaging, 115 ℃ high pressure sterilization for 30 minutes, and set aside.