抗生素檢定培養(yǎng)基Ⅵ號配方
發(fā)布時間：
2022-12-27
作者：
抗生素檢定培養(yǎng)基Ⅵ號英文名稱：Antibiotic Assay MediumⅥ
產(chǎn)品編號：11810
產(chǎn)品規(guī)格：250g/瓶
產(chǎn)品用途：用于粘菌素效價的測定
Formula(抗生素檢定培養(yǎng)基Ⅵ號配方)： g/L
Peptone（蛋白胨） 8.0g
Beef Extract（牛肉浸出粉） 3.0g
Yeast Extract（酵母浸出粉) 5.0g
Monopotassium phosphate（磷酸二氫鉀） 1.0g
Dipotassium phosphate （磷酸氫二鉀） 3.3g
Sodium Chloride（氯化鈉） 45.0g
Dextrose（葡萄糖) 2.5g
Agar（瓊脂） 15.0g
pH7.3±0.1(25℃)
培養(yǎng)基的配制方法:
稱取本品82.8g于1L蒸餾水或去離子水中,加熱攪拌煮沸至完全溶解,分裝,115℃
高壓滅菌30分鐘,滅菌結(jié)束后請搖勻,以防瓊脂沉積于器皿底部而凝固，備用。
English name: Antibiotic Assay Medium Ⅵ
Product number: 11810
Product specification: 250g/ bottle
Usage: used for the determination of colistin titer
Formula: g/L
Peptone (Peptone) 8.0g
Beef Extract 3.0g
Calcium Extract (Yeast Extract leaching powder) 5.0g
Monopotassium phosphate (KDP) 1.0 g
Dipotassium phosphate, potassium hydrogen phosphate) 3.3 g
Sodium Chloride (nacl) 45.0 g
Dextrose 2.5g
Agar (Agar) 15.0g
PH7.3 + / - 0.1 (25 ℃)
Preparation method:
According to take this product 82.8 g in 1 l of distilled water or deionized water, heating boiling stir until completely dissolved, repackaging, 115 ℃
High pressure sterilization for 30 minutes, shake well after sterilization to prevent agar-agar deposition at the bottom of the container and solidify. Set aside.
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