強化梭菌瓊脂的配置方法
發(fā)布時間:
2022-12-27
作者:
一次性成品培養(yǎng)基生產公司 日水生物
強化梭菌瓊脂百科,英文名Reinforced Clostridium Agar,微生物檢測中用于梭菌的分離培養(yǎng);Cat. No. 11030,250g,信息首發(fā)日水生物
強化梭菌瓊脂配制方法:
稱取本品50.0g于1L蒸餾水或去離子水中,加熱煮沸至完全溶解,分裝。121℃高壓滅菌15分鐘,冷卻至50-55℃,視情況每升可添加0.02g多粘菌素B。
強化梭菌瓊脂配方 克/升
蛋白胨10.0g
牛肉粉10.0g
酵母粉3.0g
葡萄糖5.0g
可溶性淀粉1.0g
醋酸鈉3.0g
氯化鈉5.0g
L-半胱氨酸鹽酸鹽0.5g
瓊脂12.5g
pH6.8±0.2(25℃)
Preparation method of enhanced Clostridium AGAR:
Weigh 50.0g of the product in 1L distilled water or deionized water, heat and boil until completely dissolved, then pack into separate containers. Pressure sterilizing at 121℃ for 15 minutes, cooling to 50-55℃, 0.02g polymyxins B can be added per liter as appropriate.
Reinforced Clostridium Agar Formula,g/L
Peptone 10.0g,Beef Extract 10.0g,Yeast Extract 3.0g,Dextrose 5.0g,Soluble Starch 1.0g,Sodium Acetate 3.0g,Sodium Chloride 5.0g,L-Cysteine monohydrochloride 0.5g ,Agar 12.5g,pH6.8±0.2(25℃),