氯化鈉蔗糖瓊脂培養(yǎng)基的配制方法
發(fā)布時間：
2022-12-27
作者：
培養(yǎng)基哪里有得買_日水生物
氯化鈉蔗糖瓊脂培養(yǎng)基英文名Sodium Chloride Sucrose Agar Medium，用于副溶血性弧菌的分離培養(yǎng)；信息來自日水生物。編號Cat. No. 10520
氯化鈉蔗糖瓊脂培養(yǎng)基配方 克/升
蛋白胨 10.0克
蔗糖 10.0克
牛肉粉 10.0克
氯化鈉 50.0克
溴麝香草酚藍 0.04克
瓊脂 18.0克
pH7.8±0.1（25攝氏度）
氯化鈉蔗糖瓊脂培養(yǎng)基配制方法:
稱取本品98.0克于1升蒸餾水或去離子水中（可按比例增加或減少配制量）,加熱煮沸至完全溶解,分裝,121攝氏度高壓滅菌15分鐘,滅菌結(jié)束后請搖勻,以防瓊脂沉積于器皿底部而凝固,備用。
Sodium Chloride Sucrose Agar Medium Formula g/L
Peptone 10.0g,Sucrose 10.0g,Beef Extract 10.0g,Sodium Chloride 50.0g,Bromthymol Blue 0.04g,Agar 18.0g,pH7.8±0.1（25℃）
Preparation method of sodium chloride sucrose AGAR medium:
Weigh 98.0g of the product in 1L distilled water or deionized water (the amount of preparation can be increased or decreased in proportion), boil the product until it is completely dissolved, divide it into separate containers, and autoclect it at 121℃ for 15 minutes. Shake well after sterilization, to prevent AGAR deposition on the bottom of the vessel and solidify for later use.