去氧膽酸鹽瓊脂配置及成分說明
發(fā)布時(shí)間:
2022-12-27
作者:
山東培養(yǎng)基公司日水生物
去氧膽酸鹽瓊脂成分配方:g/L
蛋白胨:10.0g
去氧膽酸鈉:1.0g
磷酸氫二鉀:2.0g
檸檬酸鐵:1.0g
檸檬酸鈉:1.0g
中性紅:0.03g
氯化鈉:5.0g
乳糖:10.0g
瓊脂:15.0g
pH7.3±0.2(25℃)
去氧膽酸鹽瓊脂配制方法:稱取本品45.0g于1L蒸餾水或去離子水中,攪拌煮沸溶解1分鐘,冷卻至50—55℃傾注平板。
Formula:g/L
Peptone:10.0g
Deoxysodium Cholate:1.0g
Dipotassium Phosphate:2.0g
Ferric Citrate:1.0g
Sodium Citrate:1.0g
Neutral Red:0.03g
Sodium Chloride:5.0g
Lactose:10.0g
Agar:15.0g
pH7.3±0.2(25℃)
Preparation method of deoxycholate agar: 45.0g in 1L distilled water or deionized water, stirring, boiling and dissolving for 1 minute, cooling to 50 - 55 C pouring plate.