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木糖賴氨酸去氧膽酸鹽瓊脂的配制方法

發(fā)布時間:

2022-12-27

作者:

山東培養(yǎng)基公司日水


木糖賴氨酸去氧膽酸鹽瓊脂的配制方法:稱取本品55.2g于1L蒸餾水或去離子水中(可按比例增加或減少配制量),攪拌煮沸1分鐘使完全溶解,冷卻至50—55℃傾注平皿。
 
配方: g/L
 
酵母浸粉:3.0g
L-賴氨酸鹽酸鹽:5.0g
木糖:3.5g
乳糖:7.5g
蔗糖:7.5g
去氧膽酸鈉:2.5g
檸檬酸鐵銨:0.8g
硫代硫酸鈉:6.8g
氯化鈉:5.0g
酚紅:0.08g
瓊脂:13.5g
pH7.4±0.2(25℃)
The preparation method of xylose lysine deoxycholate agar: This product is called 55.2g in 1L distilled water or deionized water (can be increased or reduced proportionately), stirring and boiling for 1 minutes to completely dissolve, cooling to 50 - 55 degrees centigrade.
Formula:g/L
Yeast extract: 3.0g
L- lysine hydrochloride: 5.0g
Xylose: 3.5g
Lactose: 7.5G
Sucrose: 7.5G
Sodium deoxycholate: 2.5G
Ferric citrate: 0.8g
Sodium thiosulfate: 6.8g
Sodium chloride: 5.0g
Phenol red: 0.08g
Agar: 13.5g
PH7.4 + 0.2 (25 C)