酪胨大豆卵磷脂吐溫20液體培養(yǎng)基基礎的配方
發(fā)布時間：
2022-12-27
作者：
日水生物網(wǎng)站
酪胨-大豆卵磷脂-吐溫20液體培養(yǎng)基基礎配制方法：稱取本品25.0g于960ml蒸餾水或去離子水中[可按比例增加或減少配制量]，每96ml培養(yǎng)基中添加1支20106[吐溫20],48-50℃水浴30分鐘溶解，分裝，121℃高壓滅菌15分鐘，備用。
Fluid Casein Digest-Soy Lecithin-Polysorbate 20 Medium Base Formula[配方]： g/L
Pancreatic Digest of Casein[胰酪蛋白胨]：20.0g
Lecithin[卵磷脂]：5.0g
pH7.3±0.2
The basic preparation method of casein-soybean lecithin-Tween 20 liquid medium: weigh 25.0 g in 960 ml distilled water or deionized water [can increase or reduce the amount of preparation in proportion], add 1 2006 [Tween 20], dissolve in water bath at 48-50 centigrade for 30 minutes, separate and sterilize at 121 centigrade for 15 minutes.
Fluid Casein Digest-Soy Lecithin-Polysorbate 20 Medium Base Formula[formula]:g/L
Pancreatic Digest of Casein[casein peptone]:20.0g
Lecithin[lecithin]:5.0g
PH7.3 + 0.2