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胰蛋白胨膽汁瓊脂TBA粉劑的配置

發(fā)布時(shí)間:

2022-12-27

作者:

山東培養(yǎng)基公司日水生物


胰蛋白胨膽汁瓊脂(TBA)配制方法:稱取本品36.5g于1L蒸餾水或去離子水中,加熱溶解,分裝,121℃高壓滅菌15分鐘,滅菌結(jié)束后請(qǐng)搖勻,以防瓊脂沉積于器皿底部而凝固,備用。(資料來自:青島日水生物
 
配方: g/L
胰蛋白胨:20.0g
3號(hào)膽鹽:1.5g
瓊脂:15.0g
pH7.2±0.2(25℃)
The preparation method of pancreatic peptone Bile Agar (TBA): call this product 36.5g in 1L distilled water or deionized water, heat and dissolve, pack, sterilize at 121 centigrade for 15 minutes, and shake well after sterilizing, in order to prevent agar from depositing at the bottom of the vessel and set aside.
Formula: g/L
Tryptone: 20.0g
No. 3 bile salt: 1.5g
Agar: 15.0g
PH7.2 + 0.2 (25 C)