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半固體瓊脂干粉粉劑的配置

發(fā)布時(shí)間:

2022-12-27

作者:

日水生物


半固體瓊脂干粉粉劑的配制方法: 稱(chēng)取本品22.0g于1L蒸餾水或去離子水中(可按比例增加或減少配制量),加入加熱煮沸至完全溶解,分裝,121℃高壓滅菌15分鐘,備用。
 
配方: g/L
 
牛肉浸粉:3.0g
蛋白胨:10.0g
氯化鈉:5.0g
瓊脂:4.0g
pH7.4±0.2(25℃)
The preparation method of semisolid agar dry powder: This product is called 22.0g in 1L distilled water or deionized water (can increase or reduce the amount of preparation according to proportion). Heating and boiling to complete dissolving, packing, sterilizing at 121 centigrade for 15 minutes, spare.
Formula: g/L
Beef dipping: 3.0g
Peptone: 10.0g
Sodium chloride: 5.0g
Agar: 4.0g
PH7.4 + 0.2 (25 C)