緩沖蛋白胨水BPW的制備說明
發(fā)布時間：
2022-12-27
作者：
日水生物
緩沖蛋白胨水（BPW）培養(yǎng)基配制方法: 稱取本品25.5g于1L蒸餾水或去離子水中（可按比例增加或減少配制量），加熱至完全溶解，分裝，121℃高壓滅菌15分鐘,備用。
Formula/配方： g/L
Peptone/蛋白胨：10.0g
Sodium Chloride/氯化鈉：5.0g
Disodium Hydrogen Phosphate/磷酸氫二鈉：9.0g
Monopotassium Phosphate/磷酸二氫鉀：1.5g
pH7.2±0.2/25℃
Buffer peptone water (BPW)Preparation method: the product is called 25.5g in 1L distilled water or deionized water (can be increased or reduced proportionately), heated to complete dissolving, loading, sterilization at 121 centigrade for 15 minutes, and spare.
Formula: g/L
Peptone: 10.0g
Sodium chloride: 5.0g
Two sodium hydrogen phosphate: 9.0g
Potassium dihydrogen phosphate: 1.5g
PH7.2 + 0.2/25 C