膽汁七葉苷瓊脂的配制方法是什么？
發(fā)布時間：
2022-12-27
作者：
日水生物
膽汁七葉苷瓊脂干粉培養(yǎng)基配制方法:
稱取本品44.5g于1L蒸餾水或去離子水中,加熱煮沸至完全溶解，分裝，121℃高壓滅菌15分鐘，滅菌結(jié)束后請搖勻，以防瓊脂沉積于器皿底部而凝固，備用。
Formula膽汁七葉苷瓊脂配方： g/L
Peptone蛋白胨：8.0g
Bile Salts膽鹽：20.0g
Ferric Citrate檸檬酸鐵：0.5g
Aesculin七葉苷： 1.0g
Agar(瓊脂：15.0g
pH7.1±0.2(25℃）
Preparation method:
Call this product 44.5g in 1L distilled water or deionized water, heat and boil to completely dissolve, pack, sterilize at 121 centigrade for 15 minutes, after sterilization, please shake well in order to prevent agar from depositing at the bottom of the utensils.
Formula formula: g/L
Peptone peptone: 8.0g
Bile Salts bile salt: 20.0g
Ferric Citrate iron citrate: 0.5g
Aesculin seven leaf glucoside: 1.0g
Agar (agar: 15.0g)
PH7.1 + 0.2 (25 C)