日水伊紅美藍(lán)瓊脂干粉的配制方法
發(fā)布時(shí)間：
2022-12-27
作者：
日水生物
伊紅美藍(lán)瓊脂配制方法:
稱取本品39.4g于1L蒸餾水或去離子水中，加熱煮沸至完全溶解，分裝，121℃高壓滅菌15分鐘,滅菌結(jié)束后請搖勻，以防瓊脂沉積于器皿底部而凝固，備用。
Formula(伊紅美藍(lán)瓊脂配方)： g/L
Peptone（蛋白胨 ） 10.0g
Lactose (乳糖) 10.0g
Dipotassium phosphate (磷酸氫二鉀) 2.0g
Eosin Y (伊紅Y) 0.4g
Methylene Blue(美藍(lán)) 0.065g
Agar(瓊脂) 17.0g
pH7.1±0.2(25℃)
Preparation method:
According to take this product 39.4 g in 1 l of distilled water or deionized water, heat to boil until completely dissolved, repackaging, 121 ℃ high pressure sterilization for 15 minutes, please shake after sterilization, to prevent AGAR deposited on the bottom of the vessel and solidification, and set aside.
Formula: g/L
Peptone (Peptone) 10.0g
Lactose (Lactose) 10.0g
Dipotassium phosphate, potassium hydrogen phosphate) 2.0 g
Eosin Y (Eosin Y) 0.4g
Methindependence Blue 0.065g
Agar(Agar) 17.0g
PH7.1 + / - 0.2 (25 ℃)