伊紅美藍(lán)瓊脂（EMB）的成分制備
發(fā)布時(shí)間：
2022-12-27
作者：
日水生物
伊紅美藍(lán)瓊脂（EMB）的配制方法:稱取本品36.0g于1L蒸餾水或去離子水中（可按比例增加或減少配制量），加熱煮沸至完全溶解,分裝,121℃高壓滅菌15分鐘,滅菌結(jié)束后請搖勻，以防瓊脂沉積于器皿底部而凝固，備用。
Formula(配方)： g/L
Peptone\蛋白胨：10.0g
Lactose\乳糖：5.0g
Sucrose\蔗糖：5.0g
Dipotassium phosphate\磷酸氫二鉀：2.0g
Eosin Y\伊紅Y：0.4g
Methylene Blue\美藍(lán)：0.065g
Agar\瓊脂：13.5g
pH7.1±0.2(25℃)
The preparation method of eosin Blue Agar (EMB): call this product 36.0g in 1L distilled water or deionized water (can increase or reduce the dosage in proportion), heat and boil to completely dissolve, pack, sterilize at 121 centigrade for 15 minutes, after sterilization, please shake well in order to prevent agar from depositing at the bottom of the utensils.
Formula (formula):g/L
Peptone: 10.0g
Lactose: 5.0g
Sucrose: 5.0g
Two potassium hydrogen phosphate: 2.0g
Eosin Y:0.4g
Meilan: 0.065g
Agar: 13.5g
PH7.1 + 0.2 (25 C)