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馬鈴薯-葡萄糖-瓊脂培養(yǎng)基配方|制備|使用

發(fā)布時間:

2022-12-27

作者:

日水生物


馬鈴薯-葡萄糖-瓊脂培養(yǎng)基 配制方法:
   稱取本品45.0g于1L蒸餾水或去離子水中(可按比例增加或減少配制量),加熱煮沸至完全溶解,分裝,121℃高壓滅菌20分鐘,滅菌結(jié)束后請搖勻,以防瓊脂沉積于器皿底部而凝固備用。
 
 
 Formula(配方): g/L
 Potato Starch(馬鈴薯淀粉)                5.0g
 Dextrose(葡萄糖)                                20.0g
 Agar(瓊脂)                                  20.0g
 pH6.0±0.2(25℃)
 

 

Preparation method:
According to take this product 45.0 g in 1 l of distilled water or deionized water (can be in proportion to increase or decrease preparation), heating boiling until completely dissolved, repackaging, 121 ℃ high pressure sterilization for 20 minutes, please shake after sterilization, to prevent AGAR deposited on the bottom of the vessel and solidification and set aside.
 
 
Formula: g/L
Potato Starch, Potato Starch) 5.0 g
Dextrose (glucose) 20.0g
Agar (Agar) 20.0g
PH6.0 + / - 0.2 (25 ℃)