胰酪大豆胨瓊脂培養(yǎng)基（TSA）的配制方法
發(fā)布時(shí)間：
2022-12-27
作者：
胰酪大豆胨瓊脂培養(yǎng)基（TSA）
英文名稱：Trypticase Soy Agar Medium
產(chǎn)品編號(hào)：12724-1
產(chǎn)品規(guī)格：250g/瓶
產(chǎn)品用途：用于菌落總數(shù)測(cè)定、菌種純化、傳代
Formula(配方)： g/L
Pancreatic Digest of Casein （胰酪胨） 15.0g
Sodium Chloride（氯化鈉) 5.0g
Enzymatic Digest of Soybean Meal（大豆木瓜蛋白酶水解物） 5.0g
Agar（瓊脂） 15.0g
pH7.3±0.2(25℃)
培養(yǎng)基的配制方法:
稱取本品40.0g于1L蒸餾水或去離子水中（可按比例增加或減少配制量），加熱攪拌煮沸至完全溶解，分裝121℃高壓滅菌15分鐘,滅菌結(jié)束后請(qǐng)搖勻，以防瓊脂沉積于器皿底部而凝固，備用
e:10.5?_x0005_b_x0016_t_x0010_ _x0003_??_x0017_??_x0001_ kerning:1.0000pt; " >1L蒸餾水或去離子水中（可按比例增加或減少配制量），微溫溶解,分裝，121℃高壓滅菌15分鐘,備用。
Trypticase Soy Agar Medium
Product number: 12724-1
Product specification: 250g/ bottle
Product USES: used for the determination of total number of colonies, purification of strains, and propagation
Formula: g/L
Pancreatic Digest of Casein (Pancreatic Casein) 15.0g
Sodium Chloride (nacl) 5.0 g
Papain hydrolysis Enzymatic Digest of Soybean Meal (Soybean) 5.0 g
Agar (Agar) 15.0g
PH7.3 + / - 0.2 (25 ℃)
Preparation method:
According to take this product 40.0 g in 1 l of distilled water or deionized water (can be in proportion to increase or decrease preparation), heating boiling stir until completely dissolved, repackaging 121 ℃ high pressure sterilization for 15 minutes, please shake after sterilization, to prevent AGAR deposited on the bottom of the vessel and solidification, and set aside
E: 10.5 ? bt ? ? ? ? kerning: 1.0000 pt; "> 1 l of distilled water or deionized water (can be in proportion to increase or decrease preparation), dissolve the lukewarm, repackaging, 121 ℃ high pressure sterilization for 15 minutes. Set aside.