大豆酪蛋白瓊脂培養(yǎng)基(TSA)的配置和使用方法
發(fā)布時(shí)間:
2022-12-27
作者:
大豆酪蛋白瓊脂培養(yǎng)基(TSA)
英文名稱:Soybean Casein Digest Agar Medium
產(chǎn)品編號(hào):12729
產(chǎn)品規(guī)格:250g/瓶
產(chǎn)品用途:用于醫(yī)藥工業(yè)潔凈室沉降菌檢測(cè)
Formula(配方): g/L
Pancreatic Digest of Casein(胰酪蛋白胨) 15.0g
Enzymatic Digest of Soybean Meal(大豆木瓜蛋白酶消化物 )5.0g
Sodium Chloride(氯化鈉) 5.0g
Agar(瓊脂) 15.0g
pH7.3±0.2(25℃)
配制方法:
稱取本品40.0g于1L蒸餾水或去離子水中(可按比例增加或減少配制量),加熱煮沸至完全溶解,分裝,121℃高壓滅菌15分鐘,滅菌結(jié)束后請(qǐng)搖勻,以防瓊脂沉積于器皿底部而凝固,備用。
Soybean casein AGAR medium (TSA)
Now, Mr. Casein Digest Agar Medium
Product number: 12729
Product specification: 250g/ bottle
Usage: used for detecting sedimentation bacteria in clean room of pharmaceutical industry
Formula: g/L
Pancreatic Digest of Casein (Pancreatic Casein) 15.0g
Enzymatic Digest of Soybean Meal, Soybean papain digestion) 5.0 g
Sodium Chloride (nacl) 5.0 g
Agar(Agar) 15.0g
PH7.3 + / - 0.2 (25 ℃)
Preparation method:
According to take this product 40.0 g in 1 l of distilled water or deionized water (can be in proportion to increase or decrease preparation), heating boiling until completely dissolved, repackaging, 121 ℃ high pressure sterilization for 15 minutes, please shake after sterilization, to prevent AGAR deposited on the bottom of the vessel and solidification, and set aside.