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大豆酪蛋白瓊脂培養(yǎng)基(TSA)的配置和使用方法

發(fā)布時(shí)間:

2022-12-27

作者:


大豆酪蛋白瓊脂培養(yǎng)基(TSA)

 英文名稱:Soybean Casein Digest Agar Medium         

產(chǎn)品編號(hào):12729

產(chǎn)品規(guī)格:250g/瓶

產(chǎn)品用途:用于醫(yī)藥工業(yè)潔凈室沉降菌檢測(cè)

Formula(配方): g/L

Pancreatic Digest of  Casein(胰酪蛋白胨)       15.0g

Enzymatic Digest of Soybean  Meal(大豆木瓜蛋白酶消化物 )5.0g

Sodium Chloride(氯化鈉)                5.0g

Agar(瓊脂)                  15.0g

pH7.3±0.2(25℃)

 

配制方法:

      稱取本品40.0g于1L蒸餾水或去離子水中(可按比例增加或減少配制量),加熱煮沸至完全溶解,分裝,121℃高壓滅菌15分鐘,滅菌結(jié)束后請(qǐng)搖勻,以防瓊脂沉積于器皿底部而凝固,備用。

 


Soybean casein AGAR medium (TSA)
 
 
 
Now, Mr. Casein Digest Agar Medium
 
Product number: 12729
 
Product specification: 250g/ bottle
 
Usage: used for detecting sedimentation bacteria in clean room of pharmaceutical industry
 
 
 
Formula: g/L
 
Pancreatic Digest of Casein (Pancreatic Casein) 15.0g
 
Enzymatic Digest of Soybean Meal, Soybean papain digestion) 5.0 g
 
Sodium Chloride (nacl) 5.0 g
 
Agar(Agar) 15.0g
 
PH7.3 + / - 0.2 (25 ℃)
 
 
 
Preparation method:
 
According to take this product 40.0 g in 1 l of distilled water or deionized water (can be in proportion to increase or decrease preparation), heating boiling until completely dissolved, repackaging, 121 ℃ high pressure sterilization for 15 minutes, please shake after sterilization, to prevent AGAR deposited on the bottom of the vessel and solidification, and set aside.