抗生素檢定培養(yǎng)基Ⅰ號(高 pH)(pH7.8-8.0)配制方法
發(fā)布時(shí)間：
2022-12-27
作者：
抗生素檢定培養(yǎng)基Ⅰ號(高 pH)(pH7.8-8.0)英文名稱：Antibiotic Agar No.1（High pH）
產(chǎn)品編號：11803
產(chǎn)品規(guī)格：250g/瓶
產(chǎn)品用途：用于鏈霉素、卡那霉素、新霉素等的效價(jià)測定
Formula(抗生素檢定培養(yǎng)基Ⅰ號(高 pH)(pH7.8-8.0)配方)： g/L
Peptone（蛋白胨） 5.0g
Beef Extract（牛肉浸出粉） 3.0g
Dipotassium Phosphate（磷酸氫二鉀) 3.0g
Agar（瓊脂） 18.0g
pH7.9±0.1(25℃）
培養(yǎng)基的配制方法:
稱取本品29.0g于1L蒸餾水或去離子水中,加熱攪拌煮沸至完全溶解,分裝,115℃高壓滅菌30分鐘,滅菌結(jié)束后請搖勻,以防瓊脂沉積于器皿底部而凝固，備用。
(1) policy Agar No.1 (High pH)
Product number: 11803
Product specification: 250g/ bottle
Purpose: to determine the titer of streptomycin, kanamycin, neomycin, etc
Formula: g/L
Peptone (Peptone) 5.0g
Beef Extract 3.0g
Dipotassium Phosphate, potassium hydrogen Phosphate) 3.0 g
Agar (Agar) 18.0g
PH7.9 + / - 0.1 (25 ℃)
Preparation method:
According to take this product 29.0 g in 1 l of distilled water or deionized water, heating boiling stir until completely dissolved, repackaging, 115 ℃ high pressure sterilization for 30 minutes, please shake after sterilization, to prevent AGAR deposited on the bottom of the vessel and solidification, and set aside.