十六烷基三甲基溴化銨培養(yǎng)基配方
發(fā)布時間：
2022-12-27
作者：
十六烷基三甲基溴化銨培養(yǎng)基英文名稱：Cetrimide Medium
產(chǎn)品編號：11705
產(chǎn)品規(guī)格：250g/瓶
產(chǎn)品用途：用于綠膿桿菌分離培養(yǎng)
Formula(十六烷基三甲基溴化銨培養(yǎng)基配方)： g/L
Peptone（蛋白胨 ） 10.0g
Beef Extract(牛肉浸粉) 3.0g
Sodium Chloride(氯化鈉) 5.0g
Cetrimide(十六烷基三甲基溴化銨） 0.3g
Agar(瓊脂) 20.0g
pH7.5±0.1（25℃）
配制方法:
稱取本品38.3g于1L蒸餾水或去離子水中（可按比例增加或減少配制量）,加熱煮沸至完全溶解,分裝,115℃高壓滅菌20分鐘,滅菌結(jié)束后請搖勻,以防瓊脂沉積于器皿底部而凝固，備用。
English name: Cetrimide Medium
Product number: 11705
Product specification: 250g/ bottle
Purpose: for isolation and culture of pseudomonas aeruginosa
Formula: g/L
Peptone (Peptone) 10.0g
Beef Extract 3.0g
Sodium Chloride (nacl) 5.0 g
Cetrimide(ammonium hexadecyl trimethyl bromide) 0.3g
Agar(Agar) 20.0g
PH7.5 + / - 0.1 (25 ℃)
Preparation method:
According to take this product 38.3 g in 1 l of distilled water or deionized water (can be in proportion to increase or decrease preparation), heating boiling until completely dissolved, repackaging, 115 ℃ high pressure sterilization for 20 minutes, please shake after sterilization, to prevent AGAR deposited on the bottom of the vessel and solidification, and set aside.