腸道菌增菌液體培養(yǎng)基的制備和配制
發(fā)布時(shí)間：
2022-12-27
作者：
英文名稱：Enterobacteria Enrichment Broth
產(chǎn)品編號(hào)：12722-1
產(chǎn)品規(guī)格：250g/瓶
產(chǎn)品用途：用于耐膽鹽革蘭陰性菌的選擇性增菌
Formula(配方)： g/L
Pancreatic Digest of Gelatin（明膠胰酶水解物） 10.0g
Dextrose (葡萄糖) 5.0g
Disodium Hydrogen Phosphate•2H2O (二水合磷酸氫二鈉) 8.0g
Monopotassium phosphate(磷酸二氫鉀) 2.0g
Bile Salts(牛膽鹽) 20.0g
Brilliant Green （亮綠） 0.015g
pH7.2±0.2 (25℃)
培養(yǎng)基的配制方法:
稱取本品45.0g于1L蒸餾水或去離子水中（可按比例增加或減少配制量），沸水浴煮沸30分鐘，冷卻至常溫備用。
Intestinal bacterial growth liquid medium
Enterobacteria Enrichment Broth
Product number: 12722-1
Product specification: 250g/ bottle
Application: it is used for selective enrichment of gallbladder salt gram-negative bacteria
Formula: g/L
Pancreatic Digest of sunlight 10.0g
Dextrose (glucose) 5.0g
Disodium Hydrogen Phosphate, 2 h2o (Disodium Hydrogen Phosphate hydrate) 8.0 g
Monopotassium phosphate (KDP) 2.0 g
Bile Salts(Bile Salts) 20.0g
Brilliant Green is 0.015 grams
PH7.2 + / - 0.2 (25 ℃)
Preparation method:
Weigh 45.0g of this product in 1L distilled water or deionized water (can increase or decrease the amount of preparation in proportion), boil in boiling water for 30 minutes, cool to room temperature and set aside.
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