36種微生物實驗用培養(yǎng)基配制
發(fā)布時間：
2022-12-27
作者：
培養(yǎng)基產(chǎn)品購買
36種微生物實驗用培養(yǎng)基配制
1.牛肉膏蛋白胨培養(yǎng)基（用于細菌培養(yǎng)）
牛肉膏3g，蛋白胨10g，NaCl 5g，水1000mL，pH7.4～7.6。
2.高氏1號培養(yǎng)基(用于放線菌培養(yǎng))
可溶性淀粉20g，KNO3 1g，NaCl 0.5g，K2HPO4?3H2O 0.5g，MgSO4?7H2O0.5g，F(xiàn)eSO4?7H2O0.01g，水1000mL，pH7.4～7.6。配制時注意：可溶性淀粉要先用冷水調勻后再加入到以上培養(yǎng)基中。
3.馬丁氏（Martin）培養(yǎng)基（用于從土壤中分離真菌）
K2HPO41g，MgSO4?7H2O0.5g，蛋白胨5g，葡萄糖10g，1/3000孟加拉紅水溶液100mL，水900mL，自然pH，121℃濕熱滅菌30min。待培養(yǎng)基融化后冷卻55～60℃時加入鏈霉素(鏈霉素含量為30μg/mL)。
4.馬鈴薯培養(yǎng)基(PDA培養(yǎng)基)(用于霉菌或酵母菌培養(yǎng))
馬鈴薯(去皮)200g，蔗糖(或葡萄糖)20g，水1000mL，配制方法如下：
將馬鈴署去皮，切成約2cm2的小塊，放入1500mL的燒杯中煮沸30min，注意用玻棒攪拌以防糊底，然后用雙層紗布過濾，取其濾液加糖，再補足至1000mL，自然pH，霉菌用蔗糖，酵母菌用葡萄糖。
5.察氏培養(yǎng)基(蔗糖硝酸鈉培養(yǎng)基)(用于霉菌培養(yǎng))
蔗糖30g，NaNO3 2g，K2HPO4 1g，MgSO4?7H2O0.5g，KCl 0.5g，F(xiàn)eSO4?7H2O0.1g，水1000mL，pH7.0～7.2。
6.Hayflik培養(yǎng)基(用于支原體培養(yǎng))
牛心消化液(或浸出液)1000mL，蛋白胨10g，NaCl 5g，瓊脂15g，pH7.8～8.0，分裝每瓶70mL，121℃濕熱滅菌15min，待冷卻至80℃左右，每70mL中加入馬血清20mL，25%鮮酵母浸出液10mL，15醋酸鉈水溶液2.5mL，青霉素G鉀鹽水溶液(20萬單位以上)0.5mL，以上混合后傾注平板。
*注意：醋酸鉈是極毒的藥品，需特別注意安全操作。
7.麥氏(McCLary)培養(yǎng)基(醋酸鈉培養(yǎng)基)
葡萄糖0.1g, KCl 0.18g，酵母膏0.25g，醋酸鈉0.82g，瓊脂l.5g，蒸餾水l00mL。溶解后分裝試管，1l5℃濕熱滅菌15min。
8.葡萄糖蛋白胨水培養(yǎng)基(用于V.P.反應和甲基紅試驗)
蛋白胨0.5g，葡萄糖0.5g，K2HPO4 0.2g，水100mL，pH7.2，1l5℃濕熱滅菌20min。
9.蛋白胨水培養(yǎng)基(用于吲哚試驗)
蛋白胨10g，NaCl 5g，水1000mL，pH7.2～7.4，121℃濕熱滅菌20min。
10.糖發(fā)酵培養(yǎng)基(用于細菌糖發(fā)酵試驗)
蛋白胨0.2g，NaCl 0.5g，K2HPO4 0.02g，水100mL，溴麝香草酚藍(1%水溶液)0.3mL，糖類lg。分別稱取蛋白胨和NaCl溶于熱水中，調pH至7.4，再加入溴麝香草酚藍(先用少量95%乙醇溶解后，再加水配成1%水溶液)，加入糖類，分裝試管，裝量4～5cm高，并倒放入一杜氏小管(管口向下，管內充滿培養(yǎng)液)。115℃濕熱滅菌20min。滅菌時注意適當延長煮沸時間，盡量把冷空氣排盡以使杜氏小管內不殘存氣泡。常用的糖類，如葡萄糖、蔗糖、甘露糖、麥芽糖、乳糖、半乳糖等(后兩種糖的用量常加大為1.5%)。
11.RCM培養(yǎng)基(強化梭菌培養(yǎng)基)、(用于厭氧菌培養(yǎng))
酵母膏3g，牛肉膏l(xiāng)0g，蛋白胨10g，可溶性淀粉lg，葡萄糖5g, 半胱氨酸鹽酸鹽0.5g，NaCl 3g，NaAc 3g，水l000mL，pH8.5，刃天青3mg/L，l2l℃濕熱滅菌30min。
12.TYA培養(yǎng)基(用于厭氧菌培養(yǎng))
葡萄糖40g，牛肉膏2g，酵母膏2g，胰蛋白胨(bacto-typetone)6g，醋酸銨3g，KH2PO4 0.5g，MgSO4?7H2O0.2g，F(xiàn)eSO4?7H2O0.01g，水1000mL， pH6.5，121℃濕熱滅菌30min。
13.玉米醪培養(yǎng)基(用于厭氧菌培養(yǎng))
玉米粉65g，自來水1000mL，混勻，煮10min成糊狀，自然pH，121℃濕熱滅菌30min。
14.中性紅培養(yǎng)基(用于厭氧菌培養(yǎng))
葡萄糖40g，胰蛋白胨6g，酵母膏2g，牛肉膏2g，醋酸銨3g，KH2PO4 5g，中性紅0.2g，MgSO4?7H2O0.2g，F(xiàn)eSO4?7H2O0.01g, 水1000mL，pH6.2，121℃濕熱滅菌30min。
15.CaCO3明膠麥芽汁培養(yǎng)基(用于厭氧菌培養(yǎng))
麥芽汁(6波美)1000mL，水1000mL，CaCO310g，明膠10g，pH6.8,121℃濕熱滅菌30min。
16.BCG牛乳培養(yǎng)基(用于乳酸發(fā)酵)
(A)溶液：
脫脂乳粉100g，水500mL，加入1.6%溴甲酚綠(B.C.G)乙醇溶液1mL，80℃滅菌20min。
(B)溶液：
酵母膏10g，水500mL，瓊脂20g, pH6.8,121℃濕熱滅菌20min。以無菌操作趁熱將(A)、(B)溶液混合均勻后倒平板。
17.乳酸菌培養(yǎng)基(用于乳酸發(fā)酵)
牛肉膏5g，酵母膏5g，蛋白胨10g，葡萄糖10g，乳糖5g，NaCl 5g，水1000mL，pH6.8，121℃濕熱滅菌20min。
18.酒精發(fā)酵培養(yǎng)基(用于酒精發(fā)酵)
蔗糖10g,MgSO4?7H2O 0.5g,NH4NO30.5g,20%豆芽汁2mL，KH2PO4 0.5g，水100mL，自然pH。
19.柯索夫培養(yǎng)基(用于鉤端螺旋體培養(yǎng))
優(yōu)質蛋白胨0.4g, NaCl 0.7g,KCl 0.02g, NaHCO3 0.01g，CaCl 0.02g，KH2PO40.09g，NaH2PO40.48g，蒸餾水500mL，無菌兔血清40mL。
制法：
除兔血清外的其余各成分混合，加熱溶解，調pH至7.2，121℃濕熱滅菌20min，待冷卻后，加入無菌兔血清，制成8%血清溶液，然后分裝試管（5～10mL/管），56℃水浴滅活lh后備用。
20.豆芽汁培養(yǎng)基
黃豆芽500g，加水1000mL，煮沸l(wèi)h，過濾后補足水分，121℃濕熱滅菌后存放備用，此即為50%的豆芽汁；
用于細菌培養(yǎng)：
10%豆芽汁200mL，葡萄糖(或蔗糖)50g，水800mL，pH7.2～7.4。
用于霉菌或酵母菌培養(yǎng)：
10%豆芽汁200mL，糖50g，水800mL，自然pH。霉菌用蔗糖，酵母菌用葡萄糖。
21.LB(Luria—Bertani)培養(yǎng)基(細菌培養(yǎng)，常在分子生物學中應用)
雙蒸餾水950mL，胰蛋白胨l0g，NaCl l0g，酵母提取物(bacto- yeast extract)5g，用lmol/L NaOH (約l mL) 調節(jié)pH值至7.0，加雙蒸餾水至總體積為1L，121℃濕熱滅菌30min。
含氨芐青霉素LB培養(yǎng)基：
待LB培養(yǎng)基滅菌后冷至50℃左右加入抗生素，至終濃度為80～100mg/L。
22.復紅亞硫酸鈉培養(yǎng)基(遠藤氏培養(yǎng)基)、(用于水體中大腸菌群測定)蛋白胨10g，牛肉浸膏5g，酵母浸膏5g，瓊脂20g，乳糖10g，K2HPO40.5g，無水亞硫酸鈉5g，5%堿性復紅乙醇溶液20mL，蒸餾水1000mL。
制作過程：
先將蛋白胨、牛肉浸膏、酵母浸膏和瓊脂加入到900mL水中，加熱溶解，再加入K2PO4，溶解后補充水至l000mL，調pH至7.2～7.4。隨后加入乳糖，混勻溶解后，于115℃濕熱滅菌20min。再稱取亞硫酸鈉至一無菌空試管中，用少許無菌水使其溶解，在水浴中煮沸10min后，立即滴加于20mL 5%堿性復紅乙醇溶液中，直至深紅色轉變?yōu)榈奂t色為止。將此混合液全部加入到上述已滅菌的并仍保持融化狀態(tài)的培養(yǎng)基中，混勻后立即倒平板，待凝固后存放冰箱備用，若顏色由淡紅變?yōu)樯罴t，則不能再用。
23.乳糖蛋白胨半固體培養(yǎng)基(用于水體中大腸菌群測定)
蛋白胨10g，牛肉浸膏5g，酵母膏5g，乳糖10g，瓊脂5g，蒸餾水1000mL，pH7.2～7.4，分裝試管(l0mL/管)，115℃濕熱滅菌20min。
24.乳糖蛋白胨培養(yǎng)液(用于多管發(fā)酵法檢測水體中大腸菌群)
蛋白胨10g，牛肉膏3g，乳糖5g，NaCl 5g，蒸餾水l000mL，1.6%溴甲酚紫乙醇溶液lmL。調pH至7.2，分裝試管(l0mL/管)，并放入倒置杜氏小管，l15℃濕熱滅菌20min。
25.三倍濃乳糖蛋白胨培養(yǎng)液(用于水體中大腸菌群測定)
將乳糖蛋白胨培養(yǎng)液中各營養(yǎng)成分以擴大3倍加入到l000mL水中，制法同上，分裝于放有倒置杜氏小管的試管中，每管5mL，1l5℃濕熱滅菌20min。
26.伊紅美藍培養(yǎng)基(EMB培養(yǎng)基)(用于水體中大腸菌群測定和細菌轉導)
蛋白胨l0g，乳糖10g，K2HPO4 2g，瓊脂25g，2%/伊紅Y(曙紅)水溶液20mL,0.5%美藍(亞甲藍)水溶液l3mL，pH7.4。
制作過程：
先將蛋白胨、乳糖、K2HPO4和瓊脂混勻，加熱溶解后，調pH至7.4，1l5℃濕熱滅菌20min，然后加入已分別滅菌的伊紅液和美藍液，充分混勻，防止產(chǎn)生氣泡。待培養(yǎng)基冷卻到50℃左右倒平皿。如培養(yǎng)基太熱會產(chǎn)生過多的凝集水，可在平板凝固后倒置存于冰箱備用。在細菌轉導實驗中用半乳糖代替乳糖，其余成分不變。
27.加倍肉湯培養(yǎng)基(用于細菌轉導)
牛肉膏6g，蛋白胨20g，NaCl 10g，水1000mL，pH7.4～7.6。
28.半固體素瓊脂(用于細菌轉導)瓊脂1g，水100mL，121℃濕熱滅菌30min。
29.豆餅斜面培養(yǎng)基(用于產(chǎn)蛋白酶霉菌菌株篩選)
豆餅100g加水5～6倍，煮出濾汁100mL，汁內加入KH2PO4 0.1%，MgSO4 0.05%,(NH4)2SO4 0.05%，可溶性淀粉2%，pH6，瓊脂2%～2.5%。
30.酪素培養(yǎng)基(用于蛋白酶菌株篩選)
分別配制A液和B液。
A液：
稱取Na2HPO4?7H2O 1.07g。干酪素4g，加適量蒸餾水，并加熱溶解。
B液：
稱取 KH2PO4 0.36g，加水溶解。A、B液混合后，加入酪素水解液0.3 mL，加瓊脂20g，最后用蒸餾水定容至1000mL。
酪素水解液的配制：
1g酪蛋白溶于堿性緩沖液中，加入1%的枯草芽孢桿菌蛋白酶25mL加水至100mL，30℃水解l h。用于配制培養(yǎng)基時，其用量為1000mL，培養(yǎng)基中加入100mL以上水解液。
31.細菌基本培養(yǎng)基(用于篩選營養(yǎng)缺陷型)
Na2HPO4?7H2O 1g，MgSO4?7H2O0.2g，葡萄糖5g，NaCl 5g，K2HPO4lg，水l000mL，pH7.0，1l5℃濕熱滅菌30min。
32.YEPD培養(yǎng)基(用于酵母原生質體融合)
酵母粉10g，蛋白胨20g，葡萄糖20g，蒸餾水1000mL，pH6.0，115℃濕熱滅菌20min。
33.YEPD高滲培養(yǎng)基(用于酵母原生質體融合)
在YEPD培養(yǎng)基中加入0.6mol/L的NaCL，3%瓊脂。
34.YNB基本培養(yǎng)基(用于酵母原生質體融合)
0.67%酵母氮堿基(YNB, 不含氨基酸，Difco),2%葡萄糖，3%瓊脂，pH6.2。
另一配方為：
葡萄糖10g(NH4)2SO4 1g，K2HPO40.125g，KHPO4 0.875g，KI 0.0001g，MgSO4?7H2O0.5g，CaCl2?2H2O0.lg，NaCl0.1g，維量元素母液lmL，維生素母液lmL(母液均按常規(guī)配制)，水1000mL，pH5.8～6.0。
35.YNB高滲基本培養(yǎng)基(用于原生質體融合)
在YNB基本培養(yǎng)基中加入0.6mol/LNaCl。
36.酚紅半固體柱狀培養(yǎng)基(用于檢查氧與菌生長的關系)
蛋白胨1g，葡萄糖10g, 玉米漿10g，瓊脂7g，水1000mL，pH7.2。在調好pH后，加入1.6%酚紅溶液數(shù)滴，至培養(yǎng)基變?yōu)樯罴t色，分裝于大試管中，裝量約為試管高度的1/2，1l5℃滅菌20min。細菌在此培養(yǎng)基中利用葡萄糖生長產(chǎn)酸，使酚紅從紅色變成黃色，在不同部位生長的細菌，可使培養(yǎng)基的相應部位顏色改變,但注意培養(yǎng)時間太長，酸可擴散以致不能正確判斷結果。
以上各種培養(yǎng)基均可配制成固體或半固體狀態(tài)，只需改變瓊脂用量即可，前者為1.5%～2.0%，后者為0.3%～0.8%。
36 kinds of microorganism were prepared by culture medium
1. Beef paste peptone culture medium (for bacterial culture)
Beef paste 3g, peptone 10g, NaCl 5g, water 1000mL, pH7.4 ~ 7.6.
2. Gaoshi 1 medium (for actinomycetes culture)
Soluble starch 20g, kno31g, NaCl 0.5g, K2HPO4?3H2O 0.5g, MgSO4? 7h2o0.5g, FeSO4? 7h2o0.1 g, water 1000mL, pH7.4 ~ 7.6. Note: soluble starch should be mixed with cold water before adding to the above medium.
3. Martin medium (used to separate fungi from soil)
K2HPO41g, MgSO4 ? 7 h2o0. 5 g, 5 g peptone, 10 g of glucose, 1/3000 Bangladesh red aqueous solution 100 ml, 900 ml water, natural pH, 121 ℃ hot and humid sterilization 30 min. After being medium melt cooling 55 ~ 60 ℃ with streptomycin (streptomycin content is 30 mu g/mL).
4. Potato culture medium (PDA)(for mold or yeast culture)
Potatoes (peeled)200g, sucrose (or glucose)20g, water 1000mL, the preparation method is as follows:
Jingle bell department, peel cut into 2 cm2 small pieces, add 1500 ml beaker boiling in 30 min, stir with a glass rod to prevent the paste, then use double gauze filter, take its filtrate sugar, up to 1000 ml, natural pH, mould with cane sugar, yeast with glucose.
5. Chace culture medium (sucrose sodium nitrate culture medium)(for mold culture)
Sucrose 30g, NaNO3 2g, K2HPO4 1g, MgSO4? 7h2o0.5g, KCl 0.5g, FeSO4? 7h2o0.1g, water 1000mL, pH7.0 ~ 7.2.
6.Hayflik medium (for mycoplasma culture)
Beef heart digestive juices (or 1000 mL leaching liquid), peptone 10 G, 5 G NaCl, AGAR, 15 G pH7.8 ~ 8.0, packing bottle of 70 mL, 121 ℃ hot and humid sterilization 15 min, stay cool to about 80 ℃, each add horse serum 20 mL to 70 mL, 25% yeast extract 10 mL, 15 thallium acetic acid aqueous solution 2.5 mL, penicillin G potassium solution (0.5 mL, more than 200000 units) blend to pour into tablets.
* note: thallium acetate is a highly toxic drug and special attention should be paid to safe operation.
7. McCLary medium (sodium acetate medium)
Glucose 0.1g, KCl 0.18g, yeast extract 0.25g, sodium acetate 0.82g, agar-agar l.5g, distilled water l00mL. Repackaging tube after dissolving, 1 l5 ℃ hot and humid sterilization for 15 min.
8. Glucose pepptone water medium (for V.P. reaction and methyl red test)
Peptone 0.5 g and 0.5 g of glucose, K2HPO4 0.5 g, water 100 ml, pH7.2, 20 min 1 l5 ℃ hot and humid sterilization.
9. Peptone water culture medium (for indole test)
Peptone 10 g, 5 g, NaCl water, 1000 ml, pH7.2 ~ 7.4, 121 ℃ hot and humid sterilization 20 min.
10. Sugar fermentation medium (for bacterial sugar fermentation test)
Peptone 0.2g, NaCl 0.5g, K2HPO4 0.02g, water 100mL, bromothymol blue (1% aqueous solution) 0.3ml, sugar lg. Weigh and peptone and NaCl respectively dissolved in hot water, the pH to 7.4, add bromothymol blue (with a small amount of 95% alcohol solution, then add water to 1% water solution), add sugar, partial shipments in vitro, charge 4 ~ 5 cm high, a duchenne and backwards into the small tube (tube, down tube filled with culture medium). 115 ℃ hot and humid sterilization 20 min. When sterilizing, pay attention to extend the boiling time properly and exhaust the cold air to keep the bubbles in the tubule. Common sugars, such as glucose, sucrose, mannose, maltose, lactose, galactose, etc.
11.RCM culture medium (enhanced clostridium culture medium), (used for anaerobic culture)
Yeast extract, 3 g beef paste l0g, peptone 10 g, soluble starch lg, glucose, 5 g, 0.5 g cysteine hydrochloride, NaCl, 3 g NaAc 3 g, water l000mL pH8.5, resazurin 3 mg/L, l2l ℃ damp heat sterilization for 30 min.
12.TYA culture medium (for anaerobic bacteria culture)
Glucose, 40 g beef paste 2 g, 2 g, yeast extract tryptone (bacto - typetone) 6 g, 3 g, ammonium acetate KH2PO4 0.5 g, MgSO4, 7 h2o0. 2 g, FeSO4, 7 h2o0. 01 g, water 1000 ml, pH6.5, 121 ℃ hot and humid sterilization 30 min.
13. Culture medium of corn mash (for anaerobic bacteria culture)
Corn flour, 65 g water, 1000 ml, blending, cook for 10 min into paste, natural pH, 121 ℃ hot and humid sterilization 30 min.
14. Neutral red medium (for anaerobic culture)
Glucose, 40 g, tryptone 6 g, 2 g yeast extract, beef extract 2 g, 3 g, ammonium acetate KH2PO4 5 g, 0.2 g, neutral red MgSO4 ? 7 h2o0. 2 g, FeSO4, 7 h2o0. 01 g, water 1000 ml, pH6.2, 121 ℃ hot and humid sterilization 30 min.
15.CaCO3 gelatin malt juice culture medium (for anaerobic bacteria culture)
Wort (6 wave beauty) 1000 ml, 1000 ml water, CaCO310g, gelatin, 10 g pH6.8, 121 ℃ hot and humid sterilization 30 min.
16.BCG milk culture medium (for lactic acid fermentation)
(A) solution:
Dried skim milk, 100 g, 500 ml water, add 1.6% bromocresol green (B.C.G) ethanol solution 1 ml, 80 ℃ sterilization 20 min.
(B) solution:
Yeast extract 10 g, water 500 ml, AGAR, 20 g pH6.8, 121 ℃ hot and humid sterilization 20 min. Mix (A) and (B) solutions thoroughly and pour over the plate.
17. Lactobacillus culture medium (for lactic acid fermentation)
Beef extract 5 g, 5 g yeast extract, peptone 10 g, 10 g of glucose, lactose, 5 g, 5 g, NaCl water 1000 ml, pH6.8, 121 ℃ hot and humid sterilization 20 min.
18. Alcoholic fermentation medium (for alcoholic fermentation)
Sucrose 10g,MgSO4?7H2O 0.5g, nh4no30.5g,20% bean sprout juice 2mL, KH2PO4 0.5g, water 100mL, natural pH.
19. Kosoff culture medium (for leptospira culture)
High-quality peptone 0.4g, NaCl 0.7g,KCl 0.02g, NaHCO3 0.01g, CaCl 0.02g, kh2po40.09g, nah2po40.48g, distilled water 500mL, aseptic rabbit serum 40mL.
Method:
Besides rabbit serum of the rest of the ingredients, cooking, adjust pH value to 7.2, 121 ℃ hot and humid sterilization 20 min, after being cooled, join the sterile rabbit serum, made from 8% serum solution, and in vitro (5 ~ 10 ml/pipe), 56 ℃ water bath inactivated lh backup.
20. Bean sprout juice culture medium
Yellow bean sprouts 500 g, 1000 ml of water, boil lh, supply water after filtration, 121 ℃ after damp heat sterilization for backup, this is for 50% of the bean sprout juice;
For bacterial culture:
10% bean sprouts juice 200mL, glucose (or sucrose)50g, water 800mL, pH7.2 ~ 7.4.
For mold or yeast culture:
10% bean sprouts juice 200mL, 50g sugar, 800mL water, natural pH. Molds use sucrose, yeast USES glucose.
21.LB(luria-bertani) medium (bacterial culture, often used in molecular biology)
Double distilled water, 950 mL, tryptone l0g, NaCl l0g, yeast extract (bacto - yeast extract), 5 g with lmol/L NaOH (about L mL) to adjust pH value to 7.0, double distilled water to the total volume of 1 L, 121 ℃ hot and humid sterilization 30 min.
LB medium containing ampicillin:
Stay LB medium after sterilization cold to 50 ℃ or so to join the antibiotics, and concentration of 80 ~ 100 mg/L.
22. The complex red (endo's medium), sodium sulfite medium (applied to determination of coliform bacteria in water) peptone 10 g, 5 g beef extract, yeast extract, 5 g, AGAR 20 g, 10 g, lactose K2HPO40. 5 g, 5 g anhydrous sodium sulfite, 5% ethanol solution of alkaline complex red 20 ml, distilled water, 1000 ml.
Production process:
Peptone, beef extract, yeast extract and AGAR AGAR were first added to 900mL water, which was heated and dissolved, followed by K2PO4. After dissolution, water was added to l000mL and pH was adjusted to 7.2 ~ 7.4. Add the lactose, blending dissolves, 20 min at 115 ℃ hot and humid sterilization. Said again take sodium sulfite to a sterile empty tube, with a few sterile water to dissolve it, after the water boil for 10 min, immediately drop founded more than 20 ml 5% ethanol solution of alkaline complex red, until turned into deep red to pale pink. And sterilized to add all the mixture to the above and still keep the melting state of medium, immediately after each tablet, after being frozen storage refrigerator spare, if the color changed from pink to scarlet, cannot reoccupy.
23. Lactose peptone semi-solid medium (for the determination of coliform flora in water)
Peptone 10 g, 5 g beef extract, yeast extract, 5 g lactose 10 g, 5 g AGAR, distilled water, 1000 ml, pH7.2 ~ 7.4, repackaging tube (l0mL/tube), 115 ℃ hot and humid sterilization 20 min.
24. Lactose peptone culture solution (for the detection of coliform bacteria in water by multi-tube fermentation)
Peptone 10g, beef paste 3g, lactose 5g, NaCl 5g, distilled water l000mL, 1.6% bromocresol violet ethanol solution lmL. Adjust pH to 7.2, repackaging tube (l0mL/tube), and into the inversion duchenne tubular, l15 ℃ hot and humid sterilization 20 min.
25. Triplose-concentrated lactose peptone culture solution (for the determination of coliform flora in water)
Will lactose peptone medium, the nourishment composition to expand 3 times, joining l000mL water method as above, packing in the test tube with our horse duchenne tubular, each tube 5 ml, 1 l5 ℃ hot and humid sterilization 20 min.
26. Erythrocyte methylene blue culture medium (EMB culture medium)(for the determination of coliforms and bacterial transduction in water)
Peptone l0g, lactose 10g, K2HPO4 2g, agar-agar 25g, 2%/ eosin Y(shu hong) aqueous solution 20mL,0.5% methylene blue (methylene blue) aqueous solution l3mL, pH7.4.
Production process:
Lactose peptone, first, K2HPO4 and AGAR blending, heating dissolves, pH 7.4, 1 l5 ℃ hot and humid sterilization 20 min, and then joined the sterilization of red liquid and blue liquid respectively, thoroughly inc