傾注平板法與斜面接種法的區(qū)別在哪里？
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2022-12-24
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傾注平板法與斜面接種法的區(qū)別在哪里？
斜面接種法：該法主要用于單個菌落的純培養(yǎng)、保存菌種或觀察細菌的某些特性。用滅菌的接種環(huán)取單個茵落或少許細菌，從培養(yǎng)基斜面底部向上劃一條直線，然后從底部向上作連續(xù)曲線劃線，一直劃到斜面頂端。
傾注平板法：測定牛乳、飲水和尿液等標本細菌數(shù)時常用此方法。將標本經適當稀釋后，取一定量加入已滅菌的平皿內，傾入已溶化并冷卻至45℃左右的定量培養(yǎng)基，混勻，待凝固后倒置、培養(yǎng)。根據(jù)培養(yǎng)基內的菌落數(shù)和稀釋倍數(shù)，即可計算出標本的細菌數(shù)。
Where is the difference between the flat plate method and the oblique inoculation method?
Inclined inoculation method: This method is mainly used for the pure culture of a single colony, preservation of bacteria or observation of certain characteristics of bacteria. A single bacteria or a few bacteria were taken from the sterilized inoculation ring, and a straight line was drawn from the bottom of the slope to the top of the slope, then a continuous curve was drawn from the bottom to the top of the slope.
Pour plate method: this method is often used to determine the number of bacteria in milk, drinking water and urine. After proper dilution, a certain amount of the sample was added to the sterilized plate, then the sample was poured into the quantitative medium which had been dissolved and cooled to about 45 C. Then the sample was mixed and inverted and cultured after coagulation. According to the number of colonies and dilution times in the culture medium, the number of bacteria in the specimen can be calculated.