TTC卵磷脂吐溫80營養(yǎng)瓊脂培養(yǎng)基基礎(chǔ)配方
發(fā)布時間：
2022-12-27
作者：
青島日水生物
TTC卵磷脂吐溫80營養(yǎng)瓊脂培養(yǎng)基基礎(chǔ)配制方法：稱取本品44.0g于1L蒸餾水或去離子水中，加入1支20107[若配制TTC卵磷脂-吐溫80營養(yǎng)瓊脂培養(yǎng)基,每100ml基礎(chǔ)中還需添加1支20101],加熱煮沸至完全溶解,分裝，121℃高壓滅菌20分鐘,滅菌結(jié)束后請搖勻，以防瓊脂沉積于器皿底部而凝固,備用。
Lecithin Tween-80 Nutrient Agar Base Formula[配方]： g/L
Peptone[蛋白胨]：20.0g
Beef Extract[牛肉浸粉]：3.0g
Lecithin[卵磷脂]：1.0g
Sodium Chloride[氯化鈉]：5.0g
Agar[瓊脂]：15.0g
pH7.3±0.1[25℃]
TTC lecithin Tween 80 nutrient agar medium preparation method: weigh 44.0g in 1L distilled water or deionized water, add a 20107 [if the preparation of TTC lecithin Tween 80 Nutrient Agar medium, every 100ml of the base also need to add a 20101], heat boiling to complete dissolution, sub-packaging, high pressure sterilization at 121 for 20 minutes, sterilization After the fungus is finished, shake well to prevent the agar from depositing on the bottom of the utensils and solidify.
Lecithin Tween-80 Nutrient Agar Base Formula[formula]:g/L
Peptone[peptone]:20.0g
Beef Extract[beef dip powder]:3.0g
Lecithin[lecithin]:1.0g
Sodium Chloride[NaCl]:5.0g
Agar[agar]:15.0g
PH7.3 + 0.1[25 C]