改良平板計(jì)數(shù)瓊脂MPCA配制說(shuō)明
發(fā)布時(shí)間：
2022-12-27
作者：
青島日水培養(yǎng)基公司
改良平板計(jì)數(shù)瓊脂配制方法：稱(chēng)取本品24.5g于1L蒸餾水或去離子水中[可按比例增加或減少配制量]，加熱煮沸至完全溶解，分裝，121℃高壓滅菌15分鐘,滅菌結(jié)束后請(qǐng)搖勻，以防瓊脂沉積于器皿底部而凝固，備用。
modifiedplatecount agar Formula[配方]： g/L
Tryptone [胰蛋白胨]：5.0g
Yeast Extract[酵母浸粉]：2.5g
Dextrose[葡萄糖]：1.0g
Defatted milk powder[脫脂奶粉]：1.0g
Agar[瓊脂] ：15.0g
pH7.0±0.2[25℃]
Modified plate counting agar preparation method: weigh this product 24.5g in 1L distilled water or deionized water [can increase or reduce the amount of preparation according to proportion], heat and boil until completely dissolved, pack, high pressure sterilization at 121 C for 15 minutes, shake well after sterilization, in order to prevent agar deposition in the bottom of the vessel and solidification, reserve.
Modifiedplatecount agar Formula[formula]:g/L
Tryptone [tryptone]:5.0g
Yeast Extract[yeast extract powder]:2.5g
Dextrose[glucose]:1.0g
Defatted milk powder[skim milk powder]:1.0g
Agar[agar]:15.0g
PH7.0 + 0.2[25 C]