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pH7.2磷酸鹽緩沖液配方說(shuō)明

發(fā)布時(shí)間:

2022-12-27

作者:


pH7.2磷酸鹽緩沖液干粉培養(yǎng)基的配制方法:稱(chēng)取本品41.0g于1L蒸餾水或去離子水中,加熱煮沸至完全溶解,作為貯存液。取貯存液1.25ml,用蒸餾水稀釋至1000ml,分裝,121℃高壓滅菌15分鐘,備用。

 
Formula[配方]: g/L
Monopotassium phosphate [磷酸二氫鉀](méi) :34.0g
Sodium hydroxide[氫氧化鈉] :7.0g
pH7.2±0.1[25℃] 
The preparation method of pH 7.2 phosphate buffer dry powder culture medium: weigh this product 41.0g in 1L distilled water or deionized water, heat and boil until completely dissolved, as storage liquid. Take the storage liquid 1.25ml, dilute it with distilled water to 1000ml, separate it, and sterilize it for 15 minutes at 121 C, standby.
Formula[formula]:g/L
Monopotassium phosphate [potassium dihydrogen phosphate]:34.0g
Sodium hydroxide[sodium hydroxide]:7.0g
PH7.2 + 0.1[25 C]