大豆酪蛋白消化物瓊脂培養(yǎng)基配方
發(fā)布時間：
2022-12-27
作者：
大豆-酪蛋白消化物瓊脂培養(yǎng)基配制方法：稱取本品40.0g于1L蒸餾水或去離子水中[可按比例增加或減少配制量]，加熱煮沸至完全溶解，分裝，121℃高壓滅菌15分鐘,滅菌結(jié)束后請搖勻，以防瓊脂沉積于器皿底部而凝固，備用。
Formula[配方]： g/L
Pancreatic Digest of Casein[胰酪蛋白胨]：15.0g
Enzymatic Digest of Soybean Meal[大豆木瓜蛋白酶消化物 ] ：5.0g
Sodium Chloride[氯化鈉] ：5.0g
Agar[瓊脂] ：15.0g
pH7.3±0.2[25℃]
Soybean-casein digestive agar medium preparation method: weigh 40.0g in 1L distilled water or deionized water [can be increased or reduced in proportion to the amount of preparation], heat and boil to complete dissolution, sub-pack, high-pressure sterilization at 121 C for 15 minutes, after sterilization, shake well, in order to prevent agar deposition in the bottom of the utensil and solidify, reserve.
Formula[formula]:g/L
Pancreatic Digest of Casein[casein peptone]:15.0g
Enzymatic Digest of Soybean Meal[]:5.0g papain digest]:5.0g
Sodium Chloride[NaCl]:5.0g
Agar[agar]:15.0g
PH7.3 + 0.2[25 C]