糖發(fā)酵培養(yǎng)基基礎(chǔ)配方
發(fā)布時(shí)間：
2022-12-27
作者：
山東培養(yǎng)基公司日水生物
糖發(fā)酵培養(yǎng)基基礎(chǔ)培養(yǎng)基配制方法：稱取本品20.0g于1L蒸餾水或去離子水中[可按比例增加或減少配制量],微溫溶解，按需求加入0.5%糖溶液制備成糖發(fā)酵培養(yǎng)基，分裝，121℃高壓滅菌15分鐘,備用。
Sugar Fermentation Medium Base Formula[配方]： g/L
Peptone[蛋白胨]：10.0g
Beef Extract[牛肉浸粉]：5.0g
Sodium Chloride[氯化鈉]：3.0g
Disodium Hydrogen Phosphate [十二水磷酸氫二鈉] ：2.0g
Bromthymol Blue[溴麝香草酚藍(lán)]：0.024g
pH7.4±0.1[25℃]
Sugar fermentation medium preparation method: weigh 20.0g in 1L distilled water or deionized water [can increase or reduce the amount of preparation in proportion], slightly temperature solution, add 0.5% sugar solution as required to prepare sugar fermentation medium, sub-packed, high-pressure sterilization at 121 C for 15 minutes, ready.
Sugar Fermentation Medium Base Formula[formula]:g/L
Peptone[peptone]:10.0g
Beef Extract[beef dip powder]:5.0g
Sodium Chloride[NaCl]:3.0g
Disodium Hydrogen Phosphate [twelve sodium hydrogen phosphate two]:2.0g
Bromthymol Blue[bromothymol blue]:0.024g
PH7.4 + 0.1[25 C]