Honda氏產(chǎn)毒肉湯基礎(chǔ)配方說明
發(fā)布時間：
2022-12-27
作者：
山東培養(yǎng)基公司日水
Honda氏產(chǎn)毒肉湯基礎(chǔ)配制方法：稱取本品52.5g于1L蒸餾水或去離子水中[可按比例增加或減少配制量],微溫溶解，分裝，121℃高壓滅菌15分鐘,冷至45-50℃,每毫升培養(yǎng)基添加90ug無菌林可霉素溶液，備用。
Honda Toxin-Producing Broth Formula[配方]： g/L
Casein hydrolysate[水解酪蛋白]：20.0g
Yeast Extract[酵母浸粉]：10.0g
Sodium Chloride[氯化鈉]：2.5g
Disodium Hydrogen Phosphate[磷酸氫二鈉]：15.0g
Dextrose[葡萄糖]：5.0g
Magnesium Sulfate[硫酸鎂]：0.025g
Ferric Chloride [氯化鐵]：0.0025g
Cobalt chloride[氯化鈷]：0.01g
pH7.5±0.2[25℃]
The basic preparation method of Honda's broth producing broth: This product is called 52.5g in 1L distilled water or deionized water [can increase or reduce the amount of preparation according to proportion], microtemperature dissolving, packing, sterilization at 121 C for 15 minutes, cold to 45-50 C, and adding 90ug aseptic lincomycin solution for every milliliter medium, and spare.
Honda Toxin-Producing Broth Formula[formula]:g/L
Casein hydrolysate[hydrolyzed casein]:20.0g
Yeast Extract[yeast extract powder]:10.0g
Sodium Chloride[sodium chloride]:2.5g
Disodium Hydrogen Phosphate[hydrogen phosphate two sodium]:15.0g
Dextrose[glucose]:5.0g
Magnesium Sulfate[Magnesium Sulfate]:0.025g
Ferric Chloride [ferric chloride]:0.0025g
Cobalt chloride[cobalt chloride]:0.01g
PH7.5 + 0.2[25 C]