EC-MUG培養(yǎng)基配方
發(fā)布時間:
2022-12-27
作者:
山東培養(yǎng)基公司日水生物
EC-MUG培養(yǎng)基配制方法:稱取本品37.1g于1L蒸餾水或去離子水中(可按比例增加或減少配制量),微溫溶解,分裝試管,115℃高壓滅菌20分鐘,備用。
配方: g/L
胰蛋白胨:20.0g
氯化鈉:5.0g
3號膽鹽:1.5g
乳糖:5.0g
磷酸氫二鉀:4.0g
磷酸二氫鉀:1.5g
MUG:0.05g
pH6.9±0.2(25℃)
EC-MUG culture medium preparation method: This product is called 37.1g in 1L distilled water or deionized water (can increase or reduce the amount of preparation according to proportion), microtemperature dissolving, separation test tube, 115 centigrade high pressure sterilization for 20 minutes, reserve.
Formula: g/L
Tryptone: 20.0g
Sodium chloride: 5.0g
No. 3 bile salt: 1.5g
Lactose: 5.0g
Two potassium hydrogen phosphate: 4.0g
Potassium dihydrogen phosphate: 1.5g
MUG:0.05g
PH6.9 + 0.2 (25 C)