結(jié)晶紫中性紅膽鹽-4-甲基傘形酮-β-D-葡萄糖苷瓊脂VRBA-MUG配方
發(fā)布時(shí)間：
2022-12-27
作者：
山東培養(yǎng)基公司日水
結(jié)晶紫中性紅膽鹽-4-甲基傘形酮-β-D-葡萄糖苷瓊脂（VRBA-MUG）用于大腸桿菌平板計(jì)數(shù)。
配制方法：稱取本品44.6g于1L蒸餾水或去離子水中，攪拌煮沸溶解2分鐘，冷卻至50—55℃傾注平板。
VRBA-MUG Formula(配方)： g/L
Peptone（蛋白胨）： 7.0g
Yeast Extract（酵母浸粉）：3.0g
Lactose（乳糖） :10.0g
Sodium Chloride(氯化鈉) :5.0g
Bile Salts No.3（3號(hào)膽鹽）:1.5g
Neutral Red（中性紅）:0.03g
Crystal Violet（結(jié)晶紫）:0.002g
Agar（瓊脂）:18.0g
MUG（MUG）:0.1g
pH7.4±0.1(25℃)
Crystal violet neutral red bile salt -4- methyl umbrella shaped ketone - beta -D- glucoside agar (VRBA-MUG) was used for plate count of Escherichia coli.
Crystal violet neutral red bile salt -4- methyl paraparone - beta -D- glucoside agar preparation method: called this product 44.6g in the 1L distilled water or deionized water, stirred boiling to dissolve for 2 minutes, cooling to 50 - 55 degrees centigrade pour plate.
VRBA-MUG Formula (formula):g/L
Peptone (Dan Baidong): 7.0g
Yeast Extract (yeast extract powder): 3.0g
Lactose (lactose): 10.0g
Sodium Chloride (sodium chloride): 5.0g
Bile Salts No.3 (No. 3 bile salt): 1.5g
Neutral Red (neutral red): 0.03g
Crystal Violet (Jie Jingzi): 0.002g
Agar (Qiong Zhi): 18.0g
MUG (MUG): 0.1g
PH7.4 + 0.1 (25 C)