曙紅亞甲藍(lán)瓊脂培養(yǎng)基EMB的配置
發(fā)布時(shí)間：
2022-12-27
作者：
青島培養(yǎng)基公司日水生物
曙紅亞甲藍(lán)瓊脂培養(yǎng)基eosin methylene blue agar的配制方法：稱取本品42.5g于1L蒸餾水或去離子水中（可按比例增加或減少配制量)，加熱煮沸至完全溶解，分裝，121℃高壓滅菌15分鐘,滅菌結(jié)束后請搖勻,以防瓊脂沉積于器皿底部而凝固，備用。（資料來自：青島日水生物）
配方：g/L
蛋白胨：10.0g
乳糖：10.0g
氯化鈉：5.0g
伊紅Y：0.4g
美藍(lán)：0.065g
牛肉浸出粉：3.0g
瓊脂：14.0g
pH7.1±0.2(25℃)
The preparation method of eosin blue agar medium eosin methylene blue agar: call this product 42.5g in 1L distilled water or deionized water (can increase or reduce the dosage in proportion), heat and boil to completely dissolve, pack, sterilize at 121 centigrade for 15 minutes, and shake well after sterilization, in order to prevent agar from depositing at the bottom of the utensils Spare.
Formula: g/L
Peptone: 10.0g
Lactose: 10.0g
Sodium chloride: 5.0g
Eosin Y:0.4g
Meilan: 0.065g
Beef extract powder: 3.0g
Agar: 14.0g
PH7.1 + 0.2 (25 C)