配置Endo培養(yǎng)基干粉粉劑方法
發(fā)布時間:
2022-12-27
作者:
青島日水生物
Endo培養(yǎng)基配制方法:稱取本品3.86g于98ml蒸餾水或去離子水中并加入2ml無水乙醇(可按比例增加或減少配制量),121℃高壓滅菌15分鐘,搖勻,備用。
配方): g/L
蛋白胨:10.0g
乳糖:10.0g
磷酸氫二鉀:2.5g
亞硫酸鈉:3.3g
堿性品紅:0.3g
瓊脂:12.5g
pH7.4±0.2(25℃)
Endo culture medium preparation method: take this product 3.86g in 98ml distilled water or deionized water and add 2ml anhydrous ethanol (can increase or reduce the amount of preparation according to proportion), 121 centigrade high pressure sterilization for 15 minutes, shake up and spare.
Formula):g/L
Peptone: 10.0g
Lactose: 10.0g
Two potassium hydrogen phosphate: 2.5G
Sodium sulfite: 3.3g
Basic fuchsin: 0.3g
Agar: 12.5g
PH7.4 + 0.2 (25 C)