BPL瓊脂bpl agar微生物培養(yǎng)基干粉劑配方
發(fā)布時(shí)間：
2022-12-27
作者：
日水生物
青島日水生物BPL瓊脂配制方法:稱取本品42.0g于1L蒸餾水或去離子水中（可按比例增加或減少配制量）,加熱煮沸至完全溶解,分裝,121℃高壓滅菌15分鐘,滅菌結(jié)束后請搖勻,以防瓊脂沉積于器皿底部而凝固,備用。
Formula(配方)： g/L
肉蛋白胨：5.0g
酪蛋白胨：5.0g
肉浸粉：5.0g
氯化鈉：3.0g
磷酸氫二鈉：2.0g
乳糖：10.0g
苯酚紅：0.08g
亮綠：0.0125g
瓊脂：12.0g
pH6.9±0.2(25℃)
BPL agar preparation method: call this product 42.0g in 1L distilled water or deionized water (can increase or reduce the dosage in proportion), heat and boil to completely dissolve, pack, 121 centigrade high pressure sterilization for 15 minutes, after sterilization, please shake well in order to prevent agar from depositing at the bottom of the utensils and solidified. Spare.
Formula (formula):g/L
Meat peptone: 5.0g
Casein peptone: 5.0g
Meat dipping powder: 5.0g
Sodium chloride: 3.0g
Two sodium hydrogen phosphate: 2.0g
Lactose: 10.0g
Phenol red: 0.08g
Bright green: 0.0125g
Agar: 12.0g
PH6.9 + 0.2 (25 C)