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沙門志賀菌屬瓊脂培養(yǎng)基SS配制方法

發(fā)布時(shí)間:

2022-12-27

作者:

山東培養(yǎng)基公司日水


沙門志賀菌屬瓊脂培養(yǎng)基SS配制方法:稱取本品62.5g于1L蒸餾水或去離子水中,攪拌煮沸溶解1分鐘,冷卻至50—55℃傾注平板。
 
配方): g/L
 
蛋白胨:5.0g
牛肉浸出粉:5.0g
牛膽鹽:8.5g
乳糖:10.0g
枸櫞酸鈉: 8.5g
硫代硫酸鈉:8.5g
枸櫞酸鐵銨:1.0g
中性紅:0.025g
煌綠:0.00033g
瓊脂:16.0g
pH7.2±0.1(25℃)
The agar medium SS preparation method of Shigella Shigella is called 62.5g in 1L distilled water or deionized water, stirring and boiling to dissolve for 1 minutes, and cooling to 50 - 55 degrees centigrade.
Formula: g/L
Peptone: 5.0g
Beef extract powder: 5.0g
Bovine bile salt: 8.5g
Lactose: 10.0g
Sodium citrate: 8.5g
Sodium thiosulfate: 8.5g
Ferric citrate: 1.0g
Neutral red: 0.025g
Brilliant green: 0.00033g
Agar: 16.0g
PH7.2 + 0.1 (25 C)