沙門(mén)、志賀氏菌增菌培養(yǎng)基的配置方法
發(fā)布時(shí)間:
2022-12-27
作者:
沙門(mén)、志賀氏菌增菌培養(yǎng)基的配制方法:稱取本品50.0g于1L蒸餾水或去離子水中(可按比例增加或減少配制量),加熱煮沸,備用。
Formula(配方): g/L
Proteose\月示胨:2.0g
Peptone\蛋白胨:8.0g
Beef Extract\牛肉浸粉:3.5g
Yeast Extract\酵母浸粉:2.0g
Dextrose\葡萄糖:2.0g
Sodium Citrate\檸檬酸鈉:10.0g
Sodium Thiosulfate\硫代硫酸鈉:10.0g
Magnesium Sulfate\無(wú)水硫酸鎂:0.7g
Sodium Sulfite\無(wú)水亞硫酸鈉:0.2g
Disodium Hydrogen Phosphate\磷酸氫二鈉:4.0g
Sodium Dihydrogen Phosphate\磷酸二氫鈉:0.1g
Bile Salts\牛膽鹽:5.5g
Sodium Desoxycholate\去氧膽酸鈉:1.5g
Brilliant Green\煌綠:0.005g
pH7.1±0.1(25℃)
The preparation method of Salmen and Shigella bacteria culture medium: This product is called 50.0g in 1L distilled water or deionized water (can increase or reduce the amount of preparation according to proportion), heat and boil, spare.
Formula (formula):g/L
Peptone: 2.0g
Peptone: 8.0g
Beef dipping: 3.5g
Yeast extract: 2.0g
Glucose: 2.0g
Sodium citrate: 10.0g
Sodium thiosulfate: 10.0g
Anhydrous Magnesium Sulfate: 0.7G
Anhydrous sodium sulfite: 0.2g
Two sodium hydrogen phosphate: 4.0g
Sodium dihydrogen phosphate: 0.1g
Bovine bile salt: 5.5G
Sodium deoxycholate: 1.5g
Brilliant green: 0.005g
PH7.1 + 0.1 (25 C)