HE瓊脂培養(yǎng)基的配方
發(fā)布時間：
2022-12-27
作者：
日水生物
HE瓊脂培養(yǎng)基配制方法:稱取本品72.6g于1L蒸餾水或去離子水中（可按比例增加或減少配制量），加熱煮沸1分鐘使完全溶解，傾注平皿，備用。
配方： g/L
蛋白胨\12.0g
酵母浸粉\3.0g
蔗糖\2.0g
乳糖\12.0g
水楊素\9.0g
3號膽鹽\9.0g
氯化鈉\5.0g
硫代硫酸鈉\5.0g
檸檬酸鐵銨\1.5g
酸性品紅\0.1g
溴麝香草酚藍(lán)\0.065g
瓊脂\14.0g
pH7.5±0.2(25℃)
HE agar medium preparation method: call this product 72.6g in 1L distilled water or deionized water (can increase or reduce the amount of preparation in proportion), heat boiling for 1 minutes to completely dissolve, pour plate, spare.
Formula: g/L
Peptone \12.0g
Yeast extract \3.0g
Sucrose \2.0g
Lactose \12.0g
Salicin \9.0g
No. 3 bile salt \9.0g
Sodium chloride \5.0g
Sodium thiosulfate \5.0g
Ferric ammonium citrate \1.5g
Acid fuchsin \0.1g
Bromothymol blue \0.065g
Agar \14.0g
PH7.5 + 0.2 (25 C)